玉郎伞多糖对环磷酰胺致小鼠肝损伤的保护作用

Protective Effect of Yulangsan Polysaccharide on Liver Injury Induced by Cyclophosphamide in Mice

目的:观察玉郎伞多糖(YLSP)对环磷酰胺(CTX)致小鼠肝损伤的治疗作用并探讨其作用机制。方法:将小鼠随机分为正常对照组(NC),肝损伤模型组(CTX),阳性对照组(联苯双酯,BPDC),YLSP(玉郎伞多糖)高、中、低剂量组。除正常对照组外,其余各组小鼠每天腹腔注射CTX 40 mg·kg-1,共7 d,建立小鼠肝损伤模型。造模后连续灌胃7 d,观察YLSP对小鼠血清谷氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和丙二醛(MDA)活性,苏木精-伊红(HE)染色观察小鼠肝脏组织病理学改变。结果:与模型组比较,YLSP各剂量组均能降低小鼠血清中ALT、AST活性及肝组织中MDA的含量;同时能增加肝组织中GSH含量及SOD和GSH-Px活性(P<0.05或P<0.01);HE染色结果显示YLSP能减轻CTX所致的肝组织变性、坏死程度,可减轻小鼠肝组织病理损伤程度。结论:YLSP对CTX所致小鼠肝损伤具有良好的保护作用。

Objective：To observe the protective effect of Yulangsan polysaccharide （ YLSP） on liver injury induced by cyclophosphamide（CTX） in mice. Methods：Liver injury induced by CTX in mice was used as the animal model and the mice were randomly divided into the normal group, CTX model group, biphenyldicarboxylate （ BPDC） group, YLSP group respectively with high, medium and low dose. Except the normal group, the other groups were injected with CTX, i. p. , for 7 days to make the model. Then the animals in the YLSP groups were intragastrically administered with YLSP for 7 days. The activities of alanine aminotransferase（ ALT） , aspartate aminotransferase（ AST） in serum, malondialdehyde（ MDA） , superoxide dismutase（ SOD） , glutathione（ GSH） and glutathione peroxidase （ GSH-Px） in liver tissue were investigated. Hematoxylin and eosin （ HE） stain was used to study the changes in hepatic tissue of the pathological mice. Results：Compared with the model group, YLSP could obviously reduce the activities of ALT, AST and the content of MDA, and increase the content of GSH, SOD and GSH-Px （P〈0. 05 or P〈0. 01）. HE staining showed that YLSP had significant protective effect on liver injury induced by CTX. Conclusion：YLSP has protective effect on liver injury induced by CTX.