丹参注射液对肝性脑病的的防治作用及机制研究

Preventive and Therapeutic Effects and Underlying Mechanisms of Danshen Injections in the Treatment of Hepatic Encephalopathy

目的:探讨丹参注射液对肝性脑病(hepatic encephalopathy,HE)的防治作用及其作用的初步机制。方法:通过急性氨中毒动物模型和四氯化碳(CCl4)致肝损伤合并硫代乙酰胺(TAA)诱导髙血氨的大鼠肝性脑病模型,对氨中毒死亡时相、血氨、脑氨水平、肝脏功能以及肝脏病理组织学改变等指标进行了评价。结果:在急性氨中毒实验中,丹参注射液能使急性氨中毒小鼠存活时间明显延长;在CCL4和TAA诱导的肝性脑病大鼠模型中,丹参注射液能明显降低肝性脑病大鼠血氨和脑氨水平,并可在一定程度上降低肝性脑病大鼠血清ALT、AST、ALP、总胆红素(Tbil)含量,改善肝功能,从而防治肝性脑病。结论:丹参注射液对肝性脑病有较好的防治作用,可能与降低血氨和脑氨水平有关。

Objective：To assess the preventive and therapeutic effects and primary mechanisms of Danshen injections in the treatment of hepatic encephalopathy （ HE） . Methods：The acute ammonia poisoning model induced by ammonium chloride was established in mice, and HE model with liver injury induced by carbon tetrachlorie and high blood ammonia induced by thioacetamide was made in rats. The time of death was detected in the first model, and the histamine levels in blood and brain, liver function and liver pathological histology changes were detected respectively in the other model. Results：In the acute ammonia poisoning experiment, the time of death in Danshen injections group was longer than that in the control group. Furthermore, Danshen injections could notably decrease the histamine levels in blood and brain, reduce the levels of ALT, AST, ALP and Tbil in serum and improve the function of liver to show the preventive and therapeutic effects on HE. Conclusion：Danshen injections exhibit promising preventive and therapeutic effects on HE, which may be related to decreasing histamine levels in blood and brain.