UPLC法同时测定穿心莲内酯磺化物中2种内酯衍生物的含量

Determination of Two Andrographolide Derivatives in Andrographolide Sulfonates by UPLC

目的：建立超高效液相色谱法（UPLC）同时测定穿心莲内酯磺化物中穿心莲内酯磺化物E、穿心莲内酯磺化物F含量。方法：色谱柱：Shimadzu Shim-pack XR-ODSⅢ（2.0 m×75 mm,1.6μm）;流动相：乙睛（A）,磷酸二氢钾缓冲液（B：每1000 ml水中加入磷酸二氢钾1.361 g与庚烷-1-磺酸钠1.0 g）梯度洗脱;流速：0.4 ml·min^-1;检测波长：225 nm;柱温：30℃。结果：穿心莲内酯磺化物E在4.39-43.88 g·ml^-1（r=1.000 0）、穿心莲内酯磺化物F在4.43-44.32μg·m^l-1（r=1.000 0）范围内,呈良好的线性关系。穿心莲内酯磺化物E平均回收率为98.71%,RSD=1.41%（n=6）;穿心莲内酯磺化物F平均回收率为99.29%,RSD=0.76%（n=6）。结论：本法重复性好、灵敏度高、定量准确,可用于穿心莲内酯磺化物的质量控制。

Objective：To establish an UPLC method for the simultaneous determination of andrographolide sulfonate E and andrographolide sulfonate F in andrographolide sulfonates. Methods： The sample was separated on a Shimadzu Shim-pack XR-ODSⅢcolumn （2. 0 m × 75 mm, 1. 6 μm） with acetonitrile（ A） and potassium dihydrogen phosphate buffer solution（ B：1. 361 g potassium dihydrogen phosphate and 1. 0g heptane-1-sodium sulfonate were dissolved in 1 000 ml water） as the mobile phase with gradient elu-tion, and the flow rate was 0. 4 ml·min-1 ,the column temperature was 30℃, and the detection wavelength was set at 225nm. Results：The linear range of andrographolide sulfonate E was 4. 39-43. 88μg·ml-1（r=1. 000 0） and the average recovery was 98. 71%（RSD=1. 41%, n=6）. The linear range of andrographolide sulfonate F was 4. 43-44. 32 g·ml-1（r=1. 000 0） and the average recovery was 99. 29%（RSD=0. 76%, n=6）. Conclusion：The method is reproducible, sensitive and accurate, which can be used in the quality control of andrographolide sulfonates.