摘要
目的：拟建立用高效液相色谱法测定复方丙谷胺片中丙谷胺含量的方法。方法：采用Diamonsil C18色谱柱（250 mm×4.6 mm,5μm）,以2%醋酸铵-甲醇（40∶60）为流动相,检测波长为225 nm,外标法计算含量。结果：丙谷胺在2.60-208.10μg与峰面积有良好的线性关系,r=0.999 9,平均加样回收率为99.8%,RSD为0.5%（n=6）。结论：HPLC法测定复方丙谷胺片中丙谷胺含量准确度高,操作简单,重复性好。
Objective：To establish an HPLC method for the determination of compound proglumide tablets. Methods：A Diamonsil C18（250 mm ×4.6 mm,5 μm）column was used. The mobile phase was 2% ammonium acetate-methanol （40∶60）, the detection wavelength was 225nm, and an external standard method was employed. Results：The linear range of proglumide was 2. 60-208. 10 μg （r=0. 999 9）, and the average recovery was 99. 8%（n=6,RSD=0. 6%）. Conclusion： The HPLC determination method for compound proglumide tablets is accurate, simple and reproducible.
出处
《中国药师》
CAS
2014年第11期1967-1969,共3页
China Pharmacist
关键词
高效液相色谱法
定氮法
丙谷胺
HPLC
Nitrogen determination method
Proglumide
