血红扇头蜱和草原革蜱源性驽巴贝斯虫病原DNA检测

Detection of pathogen DNA of Babesia caballi from Rhipicephalus sanguineus and Dermacentor nuttalli

为了进一步鉴定、识别血红扇头蜱及草原革蜱的形态结构,探究来源于内地(广州某地区)的血红扇头蜱及新疆境内广泛分布的草原革蜱携带同一种病原驽巴贝斯虫的情况,试验对血红扇头蜱及草原革蜱进行扫描电镜观察、鉴定,并参考驽巴贝斯虫Bc48基因序列合成引物,提取该种蜱携带的驽巴贝斯虫病原DNA,进行PCR检测。结果表明:血红扇头蜱和草原革蜱分别在其孔区、齿式、气门板及基节外距等部位具有超微结构差异,可分别作为其同种蜱的鉴别要点。经PCR成功扩增出了血红扇头蜱和草原革蜱所携带的驽巴贝斯虫病原DNA阳性条带大小为452 bp,来源于广州某地区的血红扇头蜱携带驽巴贝斯虫的阳性率为8.16%(12/147),来源于本地的草原革蜱驽巴贝斯虫的阳性率为3.84%(6/156)。

To further identify and recognize the morphological structures of Rhipicephalus sanguineus and Dermacentor nuttalli, and explore the situation of same pathogenic Babesia caballi that carried by Rhipicephalus sanguineus from somewhere in Guangzhou and Dermacentor nuttalli widely distributed from Xinjiang in China. Rhipicephalus sanguineus and Dermacentor nuttalli were observed and identified by scanning electron microscopy; the primers were synthesized with reference to the gene sequence of Babesia cabaUi Bc48, and then the pathogen DNA of Babesia caballi carried by the ticks was extracted and used for the PCR detection. The results showed that the ultrastmctures between Rhipicephalus san- guineus and Dermacentor nuttalli were different in the porose area, dentition formula, peritreme and external spur of coxa, and so on; which could be used as the main points of identification for the same kind of ticks. 452 bp positive bands of the pathogen DNA of Babesia caballi car- ried by Rhipicephalus sanguineus and Dermacentor nuttalli, were successfully amplified by PCR; the positive rate of Babesia caballi carried by Rhipicephalus sanguineus derived from Guangzhou was 8.16% （ 12/147 ）, and the positive rate of Babesia caballi carried by Dermacentor nut- talli derived from the local was 3.84% （6/156）.