地被竹与淡竹叶提取物抗氧化活性的比较研究

Comparative analysis of antioxidant activity of bamboo leaf extracts of ground bamboo and Phyllostachysnigra var.henon

以5种地被竹与淡竹叶为材料,采用Folin试剂还原比色法、硝酸铝-亚硝酸钠比色法和DPPH法、ABTS法、FRAP法、Fenton反应法比较其提取物中总酚、总黄酮含量及其抗氧化能力。结果表明,6种竹叶均含有很高的次生代谢物,其中鹅毛竹叶提取物的提取率最高,为8.70%±0.02%,高于淡竹的提取率（8.02%±0.06%）;以干基计算,六种竹子总酚含量在4.13%－9.06%之间,总黄酮含量在2.35%－4.37%之间,其中两者含量最高的为鹅毛竹;竹叶提取物具有较强清除自由基的作用,清除DPPH自由基和ABTS自由基能力强于淡竹的有鹅毛竹和南平倭竹;不同竹种提取物的铁还原能力之间存在显著性差异,强于淡竹的只有鹅毛竹,其FRAP值为585.00μg/m L。

Six bamboo leaves（five ground bamboos and Phyllostachysnigra var） was used as the materials to analysis the contents of total phenols and total flavonoids by using Forint reduction assay reagent and aluminum nitrate-sodium nitrite colorimetry and their antioxidant activity by using DPPH,ABTS,FRAP and Fenton reaction method,respectively. The result showed that six kinds of bamboo leaves contained abundant botanic secondary metabolites. The yield of extract from Shibataea chinensis Nakai was maximum,up to 8.70%±0.02%,higher than that of extract from Phyllostachysnigra var. The yield of extract from S.nanpingensis Q.F.Zheng et K.F.Huang was 8.02%±0.06%. The content of total phenolics,on dry basis of leaves,varied in a range of 4.13% －9.06% in the six kinds of bamboo leaves,total flavonoids located in 2.35% －4.37%,respectively.Among the six bamboos,there were more contents of total phenolics and total flavonoids in the extract from Shibataea chinensis Nakai was highest. Meanwhile two species（Shibataea chinensis Nakai and S.nanpingensis Q.F.Zheng et K.F.Huang） revealed the stronger scavenging capacity against DPPH· and ABTS＋· than those of any other bamboos.The reducing power in each bamboo leaves were significantly changed（p 0.05）. The reducing power of Phyllostachysnigra var.henon was only lower than that of Shibataea chinensis Nakai,whose FRAP was 585.00μg/m L.