响应面法优化费菜多酚的提取工艺研究

Optimization of polyphenol extraction from Sedum Aizoon L.by response surface methodology

目的:利用响应面法对费菜中多酚物质的提取条件进行优化。方法:单因素实验基础上选取实验因素与水平,根据Box-Behnken Design(BBD)实验设计原理,采用三因素三水平的响应面分析法,依据回归分析确定各工艺条件的影响因素,以费菜多酚物质含量为响应值作响应面分析实验。结果:在分析各个因素的显著性和交互作用后,得出费菜多酚物质浸提的最佳工艺条件为乙醇体积分数67%,浸提温度41℃,浸提时间2h,料液比1∶25(g/m L),以焦性没食子酸为标准品,费菜多酚物质一次提取含量可达60.90mg/g。结论:响应曲面回归方程与实验结果拟合性好,此模型合理可靠,可用于实际预测。

Objective：The main research object was Sedum Aizoon L. Besides,an optimization of extraction process for from Sedum aizoon L. was established. Methods：Based on the single factor analysis,a 3-factor,3-level Box-Behnken experimental design was applied to evaluate the effect of the three factors,including ethanol concentration,ratio of material to liquid and extraction temperature. Results：The optimal extraction conditions were ethanol concentration of 67%（v/v）,extraction temperature 41℃,extraction time 2h,solid-to-liquid ratio 1∶25（w/v）. Under such extraction conditions,the experimental yield of polyphenols obtained after the first extraction was up to 60.90mg/g,which was close to the model-predicted value. Conclusion ：The established regression equation for polyphenols yield had an excellent goodness of fit. Therefore it was credible and could be used for practical prediction.