速溶芦笋粉抗焦虑活性的研究

Study on anxiolytic activity of the instant asparagus powder in rats

目的:研究速溶芦笋粉的抗焦虑作用。方法:健康雄性SD大鼠70只,按体重随机分为7组,速溶芦笋粉低、中和高剂量组(IAP-l 0.5g/kg、IAP-m 1.0g/kg、IAP-h 2.0g/kg),阳性对照酪蛋白水解物(CTH 30mg/kg)和安定(DZP 3mg/kg),空白对照组。采用慢性情绪应激模型,从行为学观察和内分泌水平角度研究速溶芦笋粉的抗焦虑作用。结果:IAP行为学观察表明:与模型对照组比较,在此焦虑模型上速溶芦笋粉的低、中剂量组受试物减少焦虑动物探究和修饰行为的动作次数,但统计结果没有显著的差异(p>0.05)。IAP内分泌水平检测结果表明:与模型对照组比较,在此焦虑模型上速溶芦笋粉的低、中和高剂量组分别显著提高小鼠血清中5-羟色氨酸(5-HT)和多巴胺(DA)的浓度(p<0.05);分别显著降低小鼠血清中皮质醇(CORT)、肾上腺素(E)和去甲肾上腺素(NA)的浓度(p<0.05)。速溶芦笋粉在给药剂量下可以显著降低15min和60min两个时间点的小鼠自主活动次数(p<0.05)。结论:速溶芦笋粉有明显抗焦虑作用。

Objective：To study anxiolytic activity of the instant asparagus powder. Methods：70 healthy SpragueDawley male rats were randomly divided into 7 groups according to their weight for daily intragastric administration of drugs：the instant asparagus powder low-dose（IAP-l 0.5g/kg）,medium-dose（IAP-m 1.0g/kg）,high-dose（IAP-h 2.0g/kg）,the positive Caseinate Hydrolyzate group（CTH 30mg/kg）,Diazepam control group（DZP 3mg/kg） and normal control group. In this test,adopting the model of chronic emotional stress,the effect of anti-anxiety of the instant asparagus powder on the behavior and endocrine level was observed. Results：The results of IAP behavior showed the instant asparagus powder low-dose group and medium-dose group had anxiolytic trend,while comparing with the normal control group in decreasing the exploration and preening behavior times of the anxiety animals,but the statistical results had no significant differences（p〈0.05）. The results of IAP endocrine level showed that on the anxiety model,the instant asparagus powder low-dose,medium-dose and high-dose could obviously increase the concentrations of 5-HT and Dopamine in rats＇ serum（p〈0.05） and also obviously decrease the concentrations of Cortisol,Epinephrine and Noradrenaline in rats＇ serum（p〈0.05）. The instant asparagus powder that were given dose could obviously decrease the the locomotor activity times of rats at two points of 15 min and 60min（p〈0.05）. Conclusion：The instant asparagus powder had obvious anti-anxiety activity.