牡蛎多糖提取及其对小鼠急性酒精肝损伤的保护作用

The extraction of oyster polysaccharide and its hepatoprotective effect against alcohol- induced hepatic injury in mice

牡蛎是我国四大养殖贝类之一,且含有丰富的糖原和多糖。利用水提醇沉法提取牡蛎多糖,通过实验研究了牡蛎多糖对小鼠急性酒精肝损伤的保护作用。实验将昆明小鼠随机平均分为6组,分别对小鼠灌胃80、160、320mg/(kg·d)不同剂量的牡蛎多糖,30d后灌胃50度乙醇造成小鼠急性肝损伤模型,以200mg/(kg·d)的联苯双酯做阳性对照,实验结束时测定血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性、乙醛脱氢酶(ALDH)水平及肝组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、乙醇脱氢酶(ADH)活性,并观察肝组织形态学变化,研究牡蛎多糖对乙醇诱导急性肝损伤小鼠的保护作用。结果表明:牡蛎多糖各剂量均能抑制肝损伤小鼠血清ALT、AST、ALDH活性的升高,且低、中、高剂量组达到显著水平,并能显著提高肝组织中SOD、ADH活性,降低MDA含量,减轻酒精对肝细胞的病理伤害。研究表明,牡蛎多糖具有保护小鼠急性酒精肝损伤的作用。

Oyster polysaccharide was prepared from ostrea gigas thunberg by water extraction and alcohol precipitation. The hepatoprotective effect of oyster polysaccharide against alcohol-induced hepatic injury in mice was studied. The Kunming mice were oral administration daily with oyster polysaccharides at three doses of 80,160,320mg/kg respectively for 30 days. Then liver injury model was established by administration of 50 degrees intragastric ethanol in mice on the 30 th day. The positive control was administrated with 200mg/kg dimethyl diphenyl bicarboxylate. The activities of serum ALT,AST and ALDH,the content of MDA and the activity of SOD,ADH in hepatic tissue were measured. The hepatic histological changes were observed by optical microscope. The results showed that oyster polysaccharides had significantly hepatoprotective activity on alcohol induced liver damage in mice. The serum AST,ALT,ALDH and liver lipid peroxides in mice administrated with oyster polysaccharides decreased significantly compared with those of experiment control.The contents of MDA and ADH in hepatic tissue were also decreased while the activities of SOD increased significantly for the mice with oyster polysaccharides compared with those of experiment control. Histopathological changes induced by alcohol were also significantly reduced by oyster polysaccharides treatment. The results indicated that oysters polysaccharides had a protective role in acute alcoholic liver injury in mice.