摘要
目的观察SnoN蛋白及TGF-β在糖尿病大鼠视网膜上的表达,探讨SnoN蛋白及TGF-β与糖尿病视网膜病变(diabetic retinopathy,DR)的关系,为DR发病机制的研究提供新的理论依据。方法选择健康雄性Wistar大鼠20只,随机分为正常对照组与糖尿病(DM)组,每组各10只。采用一次性腹腔注射50 mg·kg-1链脲佐菌素(streptozotocin,STZ)的方法建立DM大鼠模型,正常对照组一次性给予等体积柠檬酸三钠-柠檬酸缓冲液腹腔注射。分别于造模后8周和12周处死各组大鼠,均完整摘除各组大鼠的左眼球制成眼杯,采用免疫组织化学方法检测各组大鼠视网膜上SnoN蛋白及TGF-β蛋白的表达。结果造模后8周和12周,DM组大鼠血糖值分别为(22.64±3.57)mmol·L-1、(24.08±1.60)mmol·L-1,均明显高于正常对照组的(4.64±0.91)mmol·L-1、(4.50±0.66)mmol·L-1,差异均有显著统计学意义(均为P<0.01)。正常对照组大鼠视网膜上TGF-β无表达或弱表达,在造模后8周和12周时,DM组大鼠视网膜上TGF-β的OD值分别为0.121 0±0.005 6、0.155 0±0.007 0,均较正常对照组(0.022 0±0.007 9、0.025 0±0.007 0)明显增加(均为P<0.01)。正常对照组大鼠视网膜上SnoN蛋白高表达,在造模后8周和12周时,DM组大鼠视网膜上SnoN的OD值分别为0.412 0±0.011 3、0.214 0±0.006 9,均较正常对照组(0.945 0±0.007 0、0.944 0±0.005 1)明显降低(均为P<0.01),且随着病程的延长,降低更明显。结论 SnoN蛋白对TGF-β信号通路起负反馈抑制作用,能降低TGF-β信号的强度和持续时间,因此对DR的防治具有重要意义。
Objective To investigate the expression of SnoN and TGF-β in the retinas of diabetic rats,then study the role of the SnoN and TGF-β in diabetic retinopathy,and provide a new theoretical basis for studying DR pathogenesis. Methods Twenty male Wistar rats were randomly divided into normal control group and DM group,10 cases in each group. DM model was established by intraperitoneal injection of 50 mg·kg^-1STZ. At 8 weeks and 12 weeks,the rats were sacrificed and removed left eyeballs to make the eye cups. SnoN and TGF-β protein expression in the retina were detected by immunohistochemistry. Results At 8 weeks and 12 weeks,the blood glucose in DM group were（ 22. 64 ± 3. 57） mmol·L^-1and（ 24. 08 ± 1. 60） mmol·L^-1,respectively,which were higher than those in normal control group（ 4. 64 ± 0. 91） mmol·L^-1 and（ 4.50 ± 0. 66） mmol·L^-1,there were significant differences（ all P〈0. 01）. The TGF-β was ex-pressed weakly or was not expressed in rat retina of normal control group. At 8 weeks and 12 weeks,the value of average optical density of TGF-β were 0. 121 0 ± 0. 005 6 and 0. 155 0 ± 0. 007 0 in rat retina of DM group. Compared with normal control group（ 0. 022 0 ± 0.007 9 and 0. 025 0 ± 0. 007 0）,TGF-β of rat retina expression in DM group were elevated significantly（ all P〈0. 01）. The SnoN protein was expressed highly in rat retina of normal control group. At 8 weeks and 12 weeks,the value of average optical density of SnoN were 0.412 0 ± 0. 011 3 and 0. 214 0 ± 0. 006 9 in rat retina of DM group. Compared with normal control group（ 0. 945 0 ± 0. 007 0 and 0. 944 0± 0. 005 1）,SnoN of rat retina expression in DM group were reduced significantly（ all P〈0. 01）,and it was reduced furtherly with the extension of time. Conclusion The SnoN protein maybe reduce the intensity and duration of TGF-β signaling with the role of negative feedback inhibition,so has the great significance for the prevention and control of DR.
出处
《眼科新进展》
CAS
北大核心
2014年第11期1035-1037,共3页
Recent Advances in Ophthalmology
