18月龄与3月龄SD大鼠脑干Caspase-3阳性凋亡细胞的分布比较

The Distribution of Caspase-3 Positive Apoptotic Cells in 18 Months Old SD Rat's Brainstem Compared with That in 3 Months Rat

目的观察和比较老龄(18月龄)和年轻(3月龄)SD大鼠脑干Caspase-3阳性凋亡细胞的分布,研究脑干神经元在自然老化的过程中出现凋亡的分布规律。方法将健康雄性SD大鼠分为18月龄与3月龄2组,每组3只,处死后取脑干部分行石蜡包埋和连续冠状面切片,片厚6μm,每隔33张取一片,共取3套(每套30张)切片,分别行常规HE染色、免疫组织化学检测与TUNEL检测。并将获得的Caspase-3阳性结果标记到图谱上后进行三维合成。结果与3月龄大鼠相比,18月龄大鼠脑干相关区域的Caspase-3阳性细胞数量更多、分布范围更广,尤其在孤束核和脑桥网状核增加明显。结论随着大鼠年龄的增长,脑干发生细胞凋亡的区域增大、数量增多。

Objective To study whether there is apoptosis in brainstem neurons while aging by oberving the distribution of Caspase-3positive apoptotic cells in in brainstem of young and old SD rats.Methods Healthy male SD rats were divided into 2groups（3and 18month-old respectively）,3rats each group.Brainstem specimens were treated followed the brainstem＇s common paraffin embedding,sectioning and HE staining procedures（sections were6μm in thickness）.The sections were also determined by Caspase-3immunostaining and TUNEL.The Caspase-3positive cells on the rat stereotaxic atlas were drew,then composed the sections into a 3D model.ResultsCompared to 3month-old rats,there were more Caspase-3positive neurons in the brainstem and the positive neurons were distributed more extensively in 18 month-old rats spectially in nucleus of solitary tract and pontine reticular nuclei.Conclusion More neurons suffer apoptotic changes in the brainstem while aging.