复方黄甘延缓5/6肾切除大鼠慢性肾功能衰竭

Compound Huang Gan delays chronic renal failure after 5/6 nephrectomy in rats

目的观察复方黄甘对5/6肾切除慢性肾衰模型大鼠的疗效,并探讨其可能的作用机制。方法用HPLC检测复方黄甘提取物组分并定量;构建5/6肾切除大鼠慢性肾衰模型,将模型大鼠随机分为尿毒清组、科素亚组、复方黄甘低、中、高剂量组和模型组,另加假手术组共7组,每组10只大鼠,经灌胃给药12周后用全自动生化分析仪检测Scr、BUN、Ucr及24 h UPr;对残余肾组织进行HE和Masson染色观察病理变化;用RT-PCR和免疫组化检测肾组织FN、MCP-1、ICAM-1的表达。结果复方黄甘提取物中主要成分及其含量为:甘草苷(0.06%)、丹皮酚(1.06%)、芦荟大黄素(0.1%)、大黄酸(0.47%)、大黄素(0.19%)、大黄酚(0.41%)和大黄素甲醚(0.13%);复方黄甘低、中、高剂量组Scr、BUN、Ucr、Ccr及24h UPr都显著低于模型组(P<0.05);复方黄甘给药组肾小球硬化和肾间质纤维化改善较为明显,且肾组织中FN、ICAM-1 mRNA表达和蛋白表达均显著低于模型组(P<0.05)。结论复方黄甘可改善慢性肾衰模型大鼠肾功能,减轻肾小球硬化和肾间质纤维化,在一定程度上延缓慢性肾功能衰竭的进展。

Objective To observe the effect of compound Huang Gan in delaying chronic renal failure in rats after 5/6nephrectomy and explore the possible mechanisms. Methods High-performance liquid chromatography was used to was used identify the components of compound Huang Gan extract. Rat models of 5/6 nephrectomy received a 12-week treatment with intragastric administration of Niaoduqing, Cozaar, or compound Huang Gan at low, moderate or high doses（n=10）. After the treatments, the rats were sacrificed for detecting Scr, BUN, Ucr and 24 h UPr, pathological examination of the renal tissues, and determination of FN, MCP- 1, and ICAM- 1 expression levels in the renal tissues using RT- PCR and immunohistochemistry.Results The major chemical components of compound Huang Gan extract included glycyrrhizin（0.61%）, paeonol（1.2%）, aloe emodin（0.72%）, rhein（0.85%）, emodin（0.87%）, chrysophanol（0.79%） and physcion（0.8%）. Treatment with compound Huang Gan at low, moderate and high doses significantly reduced Scr, BUN, Ucr, Ccr and 24 h UPr levels（P〈0.05）, improved interstitial fibrosis and glomerulosclerosis, and reduced FN and ICAM-1 expressions（P〈0.05） in rats following nephrectomy.Conclusion Compound Huang Gan can improve the renal function and lessen glomerulosclerosis and renal interstitial fibrosis to delay the progression of chronic renal failure in rat models of 5/6 nephrectomy.