期刊文献+

Analysis of metabolic alterations in Arabidopsis following changes in the carbon dioxide and oxygen partial pressures 被引量:2

Analysis of metabolic alterations in Arabidopsis following changes in the carbon dioxide and oxygen partial pressures
原文传递
导出
摘要 As sessile organisms,plants are subject to a multitude of environmental variations including several which directly affect their interaction with the atmosphere.Given the indiscriminant nature of Rubisco,the relative rates of photosynthesis and photorespiration are known to be responsive to changes in gas composition.However,comprehensive profiling methods have not yet been applied in order to characterize the wider consequences of these changes on primary metabolism in general.Moreover,although transcriptional profiling has revealed that a subset of photorespiratory enzymes are co-expressed,whether transcriptional responses play a role in short-term responses to atmospheric compositional changes remains unknown.To address these questions,plants Arabidopsis thaliana(Arabidopsis) ecotype Columbia(Col-O) grown under normal air conditions were transferred to different CO_2 and O_2 concentrations and characterized at the physiological,molecular,and metabolic levels following this transition.The results reveal alterations in the components,which are directly involved in,or supporting,photorespiration,including transcripts and metabolite levels.The results further highlight that the majority of the regulation of these pathways is not mediated at the level of transcription and that the photorespiratory pathway is essential also in conditions in which flux through the pathway is minimized,yet suggest that flux through this pathway is not mediated at the level of transcription. As sessile organisms,plants are subject to a multitude of environmental variations including several which directly affect their interaction with the atmosphere.Given the indiscriminant nature of Rubisco,the relative rates of photosynthesis and photorespiration are known to be responsive to changes in gas composition.However,comprehensive profiling methods have not yet been applied in order to characterize the wider consequences of these changes on primary metabolism in general.Moreover,although transcriptional profiling has revealed that a subset of photorespiratory enzymes are co-expressed,whether transcriptional responses play a role in short-term responses to atmospheric compositional changes remains unknown.To address these questions,plants Arabidopsis thaliana(Arabidopsis) ecotype Columbia(Col-O) grown under normal air conditions were transferred to different CO_2 and O_2 concentrations and characterized at the physiological,molecular,and metabolic levels following this transition.The results reveal alterations in the components,which are directly involved in,or supporting,photorespiration,including transcripts and metabolite levels.The results further highlight that the majority of the regulation of these pathways is not mediated at the level of transcription and that the photorespiratory pathway is essential also in conditions in which flux through the pathway is minimized,yet suggest that flux through this pathway is not mediated at the level of transcription.
出处 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2014年第9期941-959,共19页 植物学报(英文版)
基金 supported by funding from the Max Planck 682 Society(W.L.A.,Z.N.,T.T.,and A.R.F.) the Deutsche Forschungsgemeinschaft as part of PROMICS research group 1186(A.F.,S.T.,H.B.,and A.R.F.)
关键词 Carbon dioxide partial pressures gene expression metabolic profiling oxygen partial pressures Carbon dioxide partial pressures gene expression metabolic profiling oxygen partial pressures
  • 相关文献

参考文献2

二级参考文献16

  • 1Araujo, W.L., Tohge, T., Osorio,S., Lohse, M., Balbo, I., Krahnert, I., Sienkiewicz-Porzucek, A., Usadel, B., Nunes-Nesi, A., and Fernie, A.R. (2012). Antisense inhibition of the 2-oxoglutarate dehydrogenase complex in tomato demonstrates its importance for plant respiration and during leaf senescence and fruit maturation. Plant Cell. 24, 2328-2351.
  • 2Bauwe, H., Hagemann, M., Kern, R., and Timm, S. (2012). Photorespiration has a dual origin and manifold links to central metabolism. Curro Opin. Plant Biol. 15, 269-275.
  • 3Baerenfaller, K., Grossmann, J., Grobei, M.A., Hull, R. HirschHoffmann, M., Yalovsky, 5., Zimmermann, P., Grossniklaus, U., Gruissem, W., and Baginsky, S. (2008). Genome-scale proteomics reveals Arabidopsis thaliana gene models and proteome dynamics. Science. 320, 938-941.
  • 4Boyes, D.C., Zayed, A.M., Ascenzi, R., McCaskill, A.J., Hoffman, N.E., Davis, K.R., and Gorlach, J. (2001). Growth stage-based phenotypic analysis of Arabidopsis: a model for high throughput functional genomics in plants. Plant Cell. 13, 1499-1510.
  • 5Engel, N., Ewald, R., Gupta, K.J., Zrenner, R., Hagemann, M., and Bauwe, H. (2011). The presequence of Arabidopsis serine hydroxymethyltransferase SHM2 selectively prevents import into mesophyll mitochondria. Plant Physiol. 157, 1711-1720.
  • 6Igamberdiev, A.U., Bykova, N.V., and Gardestrom, P. (1997). Involvement of cyanide-resistant and rotenone-insensitive pathways of mitochondrial electron transport during oxidation of glycine in higher plants. FEBS Lett. 412, 265-269.
  • 7Jacoby, R.P., Millar, A.H., and Taylor, N.L. (2013). Application of selected reaction monitoring mass spectrometry to field-grown crop plants to allow dissection of the molecular mechanisms of abiotic stress tolerance. Front. Plant Sci. 4:20, 10.3389/ fpls.2013.00020.
  • 8Jacoby, R.P., Taylor, N.L., and Millar, A.H. (2011). The role of mitochondrial respiration in salinity tolerance. Trends Plant Sci. 16, 614-623.
  • 9Lernmark, U., Henricson, D., Wigge, B., and Gardestrom, P. (1991). Glycine oxidation in mitochondria isolated from light grown and etiolated plant tissue. Physiol. Plant. 82, 339-344.
  • 10Mintz-Oron, S., Meir, 5., Malitsky, S., Ruppin, E., Aharoni, A., and Shlomi, T. (2012). Reconstruction of Arabidopsis metabolic network models accounting for subcellular compartmentalization and tissue-specificity. Proc. Natl Acad. Sci. USA. 109, 339-344.

共引文献2

同被引文献11

引证文献2

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部