实验性自身免疫性心肌炎小鼠体内一氧化氮的变化

Changes of nitric oxide in mice with experimental autoimmune myocarditis

目的:研究实验性自身免疫性心肌炎(experimental autoimmune myocarditis,EAM)小鼠体内一氧化氮(nitric oxide,NO)含量的变化。方法:以20只小鼠建立EAM小鼠模型(EAM组),以同数量的小鼠作为对照组。于初次免疫后21 d,观察心脏大体和组织学变化,同时提取心肌蛋白进行western blot检测。分离小鼠脾脏细胞检测巨噬细胞含量变化及细胞内NO含量,并且利用化学试剂盒对小鼠血清和心肌组织中NO含量进行分析。结果:成功建立了EAM小鼠模型。流式细胞术分析显示,EAM组小鼠脾脏中CD11b+F4/80+巨噬细胞的比率显著升高(P<0.05);但NO荧光探针检测显示,单个细胞内NO的含量明显减少(P<0.05)。与对照组相比,EAM组小鼠血清中NO的含量未见明显变化;但心脏中NO的含量显著升高(P<0.05)。Western blot的结果表明,EAM组小鼠心脏中内皮型NOS(e NOS)和诱导型NOS(i NOS)的含量均有所升高(P<0.05)。结论:NO可能是参与自身免疫性心肌炎疾病发生发展过程的一种调控物质。

AIM：To study the changes of nitric oxide （NO） in mice with experimental autoimmune myocarditis （EAM）. METHODS： A mouse model of EAM was established to observe the results at 21 days after immunization. RESULTS： Flow cytometric analysis showed that the ratio of splenic CD11b＋F4/80＋ macrophages was significantly increased in EAM mice. At the same time fluorescence probe detection demonstrated that the NO concentration in single macrophage cell was decreased. No obvious difference in NO concentration in the serum was observed between EAM mice compared to the control mice, but NO concentration in the heart of EAM mice was significantly increased compared with that in the control mice. Western blot analysis showed that endothelial nitric oxide synthase （eNOS） and inducible nitric oxide synthase （iNOS） were both increased in the heart of EAM mice. CONCLUSION： NO may be a modulatory medium in the developmental process of autoimmune myocarditis.