杉木无性系规模化组培繁育技术研究

Study on Large-scale Tissue Culture Propagation Technology of Cunninghamia lanceolata

为了建立高效的杉木规模化组培快繁体系,以杉木未木质化的春梢茎段为外植体,MS为基本培养基,研究不同生长调节剂及其浓度组合对茎段萌发和丛生芽增殖的影响。结果表明:采用未木质化的春梢茎段,剖开后匍匐放置于培养基上,腋芽容易萌发,最佳初代培方式为MS+6-BA1.0 mg/L+NAA0.5 mg/L培养基上培养10天后,转到MS+6-BA0.8 mg/L+NAA0.3 mg/L继续培养,最佳增殖培养基为MS+6-BA0.8 mg/L+NAA0.3 mg/L,无根无菌植株采用常规扦插方法生根,可成功培育杉木优良无性系苗木。

In order to establish an efficient tissue culture system for rapidly propagating Cunninghamia lanceolata, axillary buds from the segment were used as explants and cultured on MS medium. Effects of the different types and concentration of plant growth regulator on the germination of axillary buds and proliferation of multiple shoots were studied. The results showed that buds germination was easy using non-lignified spring stem segment after broken open,and then creeping placed in culture medium. The best first medium, on which the tissue was cultured for 10 days, Was MS＋6-BA1.0 mg/L＋NAA0. 5 rag/L. Then it was transferred to the medium of MS＋6-BA0. 8 mg/L＋NAA0. 3 mg/L medium, which was the best proliferation medium and its proliferation coefficient was 4.1. Non-root sterile plants could be used to cultivate excellent clones of Cunninghamia lanceolata seedlings with the traditional cutting methods.