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不同梅毒血清学检测方法的临床价值 被引量:3

Clinical value of different syphilis serological methods
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摘要 目的:评价常用的4种梅毒血清学试验检测方法在临床诊断中的价值。方法:分别用快速血浆反应素试验(RPR)、胶体金免疫层析法(GICA)、酶联免疫吸附试验(ELISA)和微粒子化学发光法(CMIA)对264例标本进行梅毒血清学试验检测,同时与明胶颗粒凝集法(TPPA)的检测结果进行比较,得出不同检测方法的敏感度和特异性。结果:RPR、GICA、ELISA、CMIA的敏感度分别为67.5%、92.1%、96.0%和98.4%,特异性分别为89.1%、100%、100%和100%。结论:RPR可结合其他方法对梅毒进行联合诊断和对已诊断的患者进行疗效观察;GICA适合快速初筛,检测结果需结合其他方法进一步确认;ELISA和CMIA均可替代TPPA法作为临床诊断试验选用。 Objective: To evaluate four kinds of commonly used serological tests for syphilis detection in the clinical diagnosis. Method: Four methods including rapid plasma reagintest(RPR), gold immunoch romatographic assay(GICA), enzyme linked immunosorbent assay(ELISA), and chemiluminescent microparticle immunoassay (CMIA)were used to detect syphilis in 264 serum specimens. The results of above different methods were com- pared with the result obtained using treponema pallidum agglutination test(TPPA) method. The false positive rate and false negative rate of the different methods were calculated. Result: The sensitivities of the four methods (RPR,GICA,ELISA, and CMIA) were 67.5%, 92.1% .96.0% and 98.4%, respectively, while the specificities were 89.1 %,100 %, 100 % and 100 %. Conclusion:RPR could be combined with other methods for the diagnosis of syphilis and do efficacy observation of diagnosed patients. GICA might be suitable for rapid screening ,while the test result need further confirmation combined with other methods. ELISA and CMIA could replace TPPA as a clinical diagnostic test method.
出处 《临床血液学杂志(输血与检验)》 CAS 2014年第5期857-859,共3页 Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)
关键词 梅毒血清学诊断 快速血浆反应素试验 胶体金免疫层析法 酶联免疫吸附试验 微粒子化学发光 明胶颗粒凝集试验 syphilis serodiagnosis rapid plasma reagintest gold immunochromatographic assay enzymelinked immunosorbent assay chemiluminescent microparticle immunoassay treponema pallidum agglutination test
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