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2例Di(a+b-)血型的血清学及PCR-SSP检测分析 被引量:1

Analysis of serology and PCR-SSP in two cases of Di(a+b-) blood group
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摘要 以往在血型血清学疑难配血中往往通过血型定型(AB0正反定型、RhDCE定型等),抗体筛查(筛选细胞、谱细胞等),抗体Coombs及吸收放散实验等步骤一般即可完成相应的配血过程。但在血清学检测条件不充分时如稀有血型抗体缺乏、谱细胞的谱型不全时等,对于稀有表型及存有高频抗原抗体的血液的配型,常规的检测方法与路径有时就显不够。此时如配合以血型基因分型检测可能的血型表型基因并判断相应的抗体存在,应当是解决问题的一个路径。我室最近在Diego系统抗体缺乏、谱细胞谱不完全的情况下,结合基因分型的方法,解决了2份存有抗高频抗原Dib抗体的定型与输血问题。 Summary It is a difficult problem to detect rare blood group and to do comptibility test between donor and patient, used the serological and genetic methods(PCR-SSP) ,to analyse the two samples which the phenotype was the group Dib--,found anti--Dib+ in them serum. at last, effectively and quickly resolved clinical emergency blood transfusion.
出处 《临床血液学杂志(输血与检验)》 CAS 2014年第5期903-905,共3页 Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)
关键词 Di(a+b-)血型 抗体筛查 序列特异性引物扩增反应 Di(a+ b-- ) bloodgroup antibody screen PCR-SSP
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参考文献3

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  • 1刘景汉,兰炯采.输血免疫血液学实验技术[M].北京:人民卫生出版社,2011:117.
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  • 8夏金晶,白浩,熊丽纹,李蓉,颜波,邵敏华,韩宝惠.高分辨率熔解曲线法在BIM基因2,903bp片段插入/缺失检测中的应用[J].中国肺癌杂志,2014,17(3):238-242. 被引量:2
  • 9曾学平,王恭,陆理,李涛,冯学冠.ABO血型A201等位基因表达A抗原活性的分析[J].临床和实验医学杂志,2014,13(23):2000-2002. 被引量:2
  • 10陈青,李平,陆乐,肖建宇,王树亚,黄成垠,姚根宏.分子生物学鉴定急性白血病患者ABO血型抗原[J].临床血液学杂志(输血与检验),2015,28(1):91-93. 被引量:7

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