摘要
为了研究稀土金属铈对黄豆种子萌发和幼苗生理活性的作用,采用不同浓度的Ce(NH4)2(NO3)6处理黄豆种子,测定种子的萌发率、种子活力、淀粉酶活性、幼苗根系活力、叶绿素含量、相对电导率(REC)以及丙二醛(MDA)含量.结果表明:(1)低浓度铈能够促进种子萌发和幼苗生长,Ce(NH4)2(NO3)6质量浓度为1.0 mg/L时,促进作用最显著,与对照组相比,萌发率增长10.2%,生长势高出3.4%,淀粉酶活性增加32.4%,根系活力提高34.3%,幼苗的REC和MDA也显著低于对照组;(2)高浓度铈(质量浓度≥50.0 mg/L)对种子萌发、根系活力具有明显抑制作用,尤其是Ce(NH4)2(NO3)质量浓度为100.0 mg/L的处理组,抑制作用最强烈.由此认为,铈对黄豆种子萌发和幼苗生长的影响为低促高抑,1.0 mg/L最能有效促进种子萌发和幼苗生长.
To investigate the influence of Cerium on the seed germination and seedlings growth of soybean, the seeds were soaked with different concentrations of Ce (NH4)2(N03)6. The parameters measured included the germination rate, the seed vigor, the amylase activity, the root activity of seedlings, the chlorophyll content, the relative conductivity (REC) and the content of Malondialdehyde (MDA). The results showed that: ( 1 ) The low concentrations of Ce can promote the germination of seed and the growth of seedlings. The mass concentration of 1.0 mg/L of Ce( NH4)2(NO3)6 had the most outstanding promoting effect. Compared with the contrast, the germination rate increased by 10.2%, the growth potential increased by 3.4%, the amylase activity increased by 32.4%, and the root activity increased by 34.3%. The REC and MDA were also apparently low er than the contrast. (2) The high concentrations of Ce (≥ 50.0 mg/L) can prohibit the seed germination and root activity, especially at the mass concentration of 100.0 mg/L of Ce (NH4)2( NO3)6. In conclusion, the low concentrations of Ce can promote the seed germination and the seedlings growth, while the high concentrations of Ce had opposite effect on soybean. The mass concentration of 1.0 mg/L of Ce(NHa)2(NO3)6 can most efficiently promote the seed germination and the seedlings growth of soybean.
出处
《天津师范大学学报(自然科学版)》
CAS
2014年第4期72-75,共4页
Journal of Tianjin Normal University:Natural Science Edition
基金
天津市自然科学基金资助项目(13JCYBJC25500)
关键词
黄豆
铈
种子萌发
淀粉酶活性
膜透性
soybean
Ce
seed germination
amylase activity
membrane permeability