无角陶赛特羊超数排卵技术研究

Study on the Superovulation Techniques in Polll Dorset Sheep

为了探索FSH剂量和不同超排处理方法对无角陶赛特羊超数排卵的效果,分别用总剂量为120IU、180IU、240IU和300IU四个剂量水平的FSH进行超数排卵。结果表明:240IU的FSH对无角陶赛特羊的超排效果最好,回收胚胎数和可用胚胎数显著高于120IU(P≤0.05),同时效果也明显优于120IU和180IU剂量组;两种超排方案FSH+PG和CIDR+FSH进行超数排卵对无角陶赛特羊排卵数、回收胚胎数以及可用胚胎数之间无显著差异(P>0.05)。

To explore the effect of superovulation with different dosages of FSH and different superovulation methods in Poll Dorset sheep, the sheep were superovulated with four different dosages of FSH, which were 120 IU, 180 IU, 240 IU and 300 IU. The results showed that the most suitable total dosages of FSH was 240 IU, The average of collected embryos number and the enable used embryos number are higher than 120 IU（P〈0.05）. The effect of 240 IU obvious better than 180 IU and 300 IU. Two superovulation methods （FSH＋PG method and CIDR＋FSH method） were used, it is found that the mean number of ovulation point, collected embryos and the enable used embryos were not different significantly in Poll Dorset sheep （P〉 0. 05）.