云南地区人乳头状瘤病毒感染与非小细胞肺癌相关性研究

Study on relationship between human papillomavirus infection and non-small cell lung cancer in Yunnan area

目的考察云南地区非小细胞肺癌(non-small cell lung cancer,NSCLC)的发生与人乳头状瘤病毒(HPV)感染的相关性,探讨HPV感染在评价NSCLC发生中的意义。方法 2012年12月-2013年9月昆明医科大学第一附属医院胸外科收治的NSCLC 50例患者,来自云南不同地区,其中确诊NSCLC患者36例、肺良性病变患者14例;选用通用型引物和HPV16、18型特异性引物,采用PCR检测仪法分别进行检查,将所有数据进行统计分析。结果 NSCLC患者中HPV16、18型呈阳性有17例,阳性率为47.2%,肺良性病变患者中呈阳性者仅为1例,阳性率为7.1%,肺良性病变患者的阳性率明显低于NSCLC患者,差异有统计学意义(P<0.05);HPV感染率在肺鳞癌中为58.3%、腺癌中为25.0%,鳞癌感染率显著高于腺癌,差异有统计学意义(P<0.05),随着肿瘤分化程度的降低,其感染率逐步增高;HPV感染率在吸烟者中为58.3%、在不吸烟者中为14.3%,吸烟者感染率显著高于腺癌,差异有统计学意义(P<0.05)。结论 HPV感染与云南地区NSCLC的发生具有一定的相关性,有可能诱导NSCLC的发生、发展,养成良好的生活习惯,如戒烟可减少HPV感染的概率,降低NSCLC的发生率,同时,HPV感染在评价NSCLC发生具有一定的借鉴意义。

OBJECTIVE To observe the relationship between the non‐small cell lung cancer （NSCLC） and the hu‐man papillomavirus （HPV） infection and explore the significance of the HPV infection in evaluation of NSCLC . METHODS A total of 50 NSCLC patients who came from various areas of Yunnan province and were treated in the thoracic surgery department of the First Affiliated Hospital of Kunming Medical University from Dec 2012 to Sep 2013 were enrolled in the study ,including 36 patients with confirmed NSCLS and 14 patients with benign lung le‐sions ;the universal primers and the HPV16 ,18‐type primers were selected ,the examination was performed by u‐sing PCR ,and all the data were statistically analyzed .RESULTS Of the NSCLC patients ,17 cases were tested pos‐itive for HPV16 ,18 type with the positive rate of 47 .2% ,and 1 case was tested positive among the patients with benign pulmonary lesion with the positive rate of 7 .1% ;the positive rate of the patients with benign pulmonary le‐sions was significantly lower than that of the patients with NSCLC ,there was statistically significant difference （P〈0 .05） .The incidence of HPV infection was 58 .3% in the patients with squamous cell carcinoma ,significant‐ly higher than 25 .0% in the patients with adenocarcinoma , there was statistically significant difference （ P〈0 .05） .With the decrease of degree of tumor differentiation ,the incidence of the infection was increased .The inci‐dence of HPV infection was 58 .3% in the patients who smoked ,14 .3% in the patients who did not ,and the in‐fection rate was significantly higher in the patients who smoked than in the patients with adenocarcinoma ,with statistical significance （P〈0 .05） .CONCLUSION The HPV infection ,to a certain extent ,is associated with the incidence of NSCLS in Yunnan area ,which may induce the outbreak and development of the NSCLC .The inci‐dence of NSCLC can be reduced through development of healthy living habits such as quitting smoking ;the HPV infection has certain reference significance in evaluation of incidence of NSCLC .