能逆转细菌耐药性的中药的筛选

Screening of Traditional Chinese Medicine That can Reverse Bacterial Drug Resistance

目的寻求能逆转细菌耐药性的中药,为缓解超级耐药菌的危害提供新思路新方法。方法射干、黄连、黄芩、大黄、二黄汤1(黄芩+大黄)、二黄汤2(黄连+大黄)、三黄汤(黄芩+黄连+大黄),煎煮法制备提取液,采取稀释法测定多重耐药的鲍曼不动杆菌对中药的MIC,以亚MIC为逆转浓度进行逆转实验,用平板稀释法检测耐药菌经中药作用前后对环丙沙星的MIC的变化判断耐药性是否逆转。进一步用碱裂解法对中药作用前后的试验菌进行质粒抽提,通过质粒图谱比对分析,判断质粒是否消除。结果射干、黄连、黄芩、大黄、二黄汤1、二黄汤2、三黄汤均有不同程度的逆转耐药作用,逆转率依次为4.2%、2.8%、6.9%、6.9%、11.1%、2.8%、11.1%,二黄汤1、三黄汤的效果更明显;质粒变化相关度(耐药逆转与质粒丢失之间的关系)依次为33.3%、100%、0%、20%、75%、50%、25%,其中黄连的质粒消除效果最佳。结论二黄汤1可能具有逆转细菌耐药性的作用,其机制可能与耐药质粒的消除有关。

To seek for some traditional Chinese medicine that can reverse bacterial drug resistance,in order to provide a new solution to the threat of super-drug resistance.METHODS Blackberrylily,Coptis,Radix Scutellariae,Rhubarb,and the compound prescription of Radix Scutellariae and Rhubarb（ named double-yellow decoction 1）,Coptis and Rhubarb（ named double-yellow decoction2）,Radix Scutellariae,Rhubarb and Coptis（ named three-yellow decoction）,which is consisting of Coptis,Radix Scutellariae and Rhubarb,were selected,then the extracts were decocted.The bacterial resistance reversal test was developed on the basis of the MIC the extracts to Acinetobacter baumannii,sub MIC as the work concentration.The MIC of ciprofloxacin was detected before and after cultured in medium containing extracts to evaluate whether the drug resistance reversed in bacteria.Then the bacteria plasmids were extracted and the electrophoresis map analyzed to identify whether some plasmids lost.RESULTS The reverse rate of Blackberrylily,Coptis,Radix Scutellariae,Rhubarb,double-yellow decoction 1,double-yellow decoction 2,three-yellow decoction was 4.2%,2.8%,6.9%,6.9%,11.1%,2.8% and 11.1%,respectively.The effect of double-yellow decoction 2 is more obvious.The relevance of plasmid was 33.3%,100%,0%,20%,75%,50% and 25%,the effect of Coptis is more obvious.CONCLUSION The extract of Coptis and Rhubarb（ double-yellow 1） is best for reversing bacterial drug resistance.