碘量法测定葡萄糖含量的小量化与绿色化研究与实践

Research and Practice of Miniaturization and Greenization of Determination of Glucose Content by Iodimetric Method

碘量法是测定葡萄糖含量的常规分析方法,也是定量分析化学实验常开设的综合性实验.对传统教材中的碘量法测定葡萄糖含量实验内容进行了小量化改造,并研究了氢氧化钠用量及滴加速度、反应温度、放置时间、酸的用量、滴定震荡速度及淀粉加入时刻等影响因素对碘量法测定葡萄糖含量的影响.实验结果表明,小量化滴定能够达到常量滴定分析要求的精密度与准确度,可大幅度降低实验消耗、节省实验经费80％以上,使学生树立绿色化学思想和环境保护意识,全面提高人才的培养质量和效益.

The iodometric method is a routine analysis method to determine glucose content and usually used in comprehensive quantitative analytical chemistry experiments. The miniaturization of the traditional iodometric method for determination of glucose content is studied, some influential factors such as amounts of sodium hydroxide, titration speed, reaction temperature, storage time, amounts of acid, shaking extent as well as the moment of addition of starch solution were researched. The experimental results showed that the miniaturized titration method may achieve the same precision and accuracy of the conventional titrimetric analysis, and satisfy the requirements of titrimetric analysis experimental teaching. At the same time, this method can greatly reduce the experimental reagent wastage, save experimental expenditure more than 80%, enable students to establish the concept of green chemistry and environmental protection awareness, and raise the quality and benefit of the talent training.