生物信息学预测hsa-miR-186的靶基因与功能

Bioinformatics prediction for target genes of hsa-miR-186 and analysis on its function

目的利用生物信息学的方法预测hsa-miR-186的靶基因与功能,为深入研究其生物学功能提供理论依据。方法利用Pubmed检索miR-186相关文章,进行总结分析;用miRbase、NCBI等在线工具分析hsa-miR-186序列及所在基因组特征;用TargetScan、PicTar及miRanda预测hsa-miR-186的靶基因,结合已证实的靶基因进行功能富集分析(GO analysis)和信号转导通路富集分析(Pathway analysis)。结果已有的研究证实miR-186与多种肿瘤发生、发展有关;参与压力应激引起的神经功能改变,并在心血管疾病中表达异常。hsa-miR-186位于人类染色体1p31.1,序列具有高度保守性。hsa-miR-186靶基因功能富集在糖代谢、肾脏发育、视觉功能、神经功能、脂质转运等生物学过程;信号通路涉及前列腺癌、肿瘤信号通路、p53信号通路、黑色素瘤、细胞黏附分子和细胞周期等信号通路。结论 hsa-miR-186功能广泛,与神经系统发育、细胞增殖与凋亡、糖代谢和脂质转运等密切相关。

Objective To predict the target gents and function of hsa-miR-186 by bioinformatics analysis and provide certain theoretical basis for the further studies. Methods The relative studies on hsa-miR-186 were retrieved from PubMed and Google. The sequence and genome characteristics of hsa-miR-186 were analyzed on line by miRbase database, NCBI, etc. The target genes of hsamiR-186 were predicted by TargetScan, PicTar and miRanda, and then demonstrated by Gene Ontology （GO） and Pathway （KEGG） Enrichment analysis. Results Hsa-miR-186 may be related to multiple tumorigenesis, neurological function and cardiovascular disease. Hsa-miR-186 located in human chromosome lp31.1 and highly conserved among different species. GO analysis demonstrated that the target genes of hsa-miR-186 might participate in the biological processes of glucose metabolism, kidney development, visual function, nerve function, lipid transport. Pathway analysis showed that these target genes were mainly involved in prostate cancer, p53 signaling pathway, melanoma, cell adhesion molecules and cell cycle signaling pathways. Conclusion Hsa-miR-26b may have extensive functions and closely relate to nervous system development, cell proliferation and apoptosis, glucose metabolism, lipid transport, etc.