微波辅助从羊肺中提取肝素钠的工艺研究

Microwave-assisted extraction technology ofheparin sodium from sheep lung

以羊肺为原料，采用微波辅助法及盐解、沉淀剂除蛋白质的方法提取和分离肝素钠。在单因素试验基础上，设计L9（3^4）正交试验确定最佳提取工艺条件。结果表明，最佳提取工艺为料液比1：15（g：mL），微波作用时间180S，微波功率480W，沉淀剂用量为0-8％，此条件下肝素钠得率为294．78mg／kg。

Using sheep lung as raw material, heparin sodium was separated and extracted by microwave-assisted method, salt-solution and precipitant deproteinization method. L9（3^4） orthognnal test was designed to determine the optimum process conditions on the basis of single factor test. The results indicated that the optimum extraction process was material liquid ratio 1：15 （g：ml）, microwave time 180 s, microwave power 480 W, precipitation agent addition 0.8%. Under these conditions, the heparin sodium yield was 294.78 mg/kg.