摘要
目的建立调经糖浆中阿魏酸的HPLC含量测定方法。方法色谱柱为Diamonsil C18（4．6mm×250mm，5μm），流动相为乙腈-1％冰乙酸溶液（18：82）为流动相，流速1．0mL·min-1，柱温为30℃，测定波长322nm。结果阿魏酸在4．95～99μg·mL-1浓度范围内线性关系良好，标准曲线为A=54．256C＋0．6693，r=0．9999。结论该方法方便、快速、准确，可用于调经糖浆的质量控制。
OBJECTIVE To establish a HPLC method for determination of Ferulie Acid in Tiaojing Syr- up. METHODS The determination was carried out on a Diamonsil C 18 （4.6mm × 250mm, 5 μm） , using the mobile phase of acetonitrile-l% （w） glacial acetic acid （18：82） at a flow rate of 1.0mL · min-1. The column temperature was at 30℃ and the detection wavelength was at 322nm. RESULTS Ferulic Acid showed a good linear relationship at range of 4.95～991μg mL-1, the linear calibration curves was A = 54. 256C ＋ 0.6693, r = 0. 9999. CONCLUSION The method is convenient, quick and accurate. Which is suitable for the quality control of Tiaojing Syrup.
出处
《海峡药学》
2014年第10期63-64,共2页
Strait Pharmaceutical Journal
