荆芥挥发油气相色谱指纹图谱研究

Study on GC Fingerprint of Volatile Oil of Herba Schizonepetae

目的:建立荆芥挥发油的气相色谱指纹图谱。方法:采用HP-INNOWAX毛细管柱(30 m×320μm,0.5μm),进样口温度为250℃,检测器温度为250℃,程序升温,初始温度为100℃,以3℃/min升至130℃,保持10 min,再以3℃/min升至160℃,再以5℃/min升至240℃。载气流速为1 mL/min,分流比为50:1。结果:建立荆芥挥发油GC指纹图谱,10批荆芥挥发油标示出23个共有峰,相似度高。结论:该方法准确可靠、重复性好,所建立的指纹图谱可作为荆芥挥发油的质量控制依据。

Objective： To establish the method of fingerprint analysis on volatile oil of Jingjie （Herba schizonepetae） by GC. Methods： The HP-INNOWAX capillary column（30 m×320 μm, 0.5 μm） was used with the sampling temperature at 250 ℃ and the FID detector temperature at 250 ℃. The temperature programming was adopted with the initial column temperature at 100 ℃, increased by 3 ℃/min to 130 ℃, after keeping for 10 min, re-increased by 3 ℃/min to 160 ℃, then re-increased by 5℃/min to 240 ℃. The flow rate of the carrier gas was 1 mL/min, with the split ratio of 50：1. Results： The GC fingerprint of the volatile oil of Jingjie （Herba schizonepetae） was established, 23 common peaks were identified in 10 batches of the Jingjie （Herba sehizonepetae）. Conclusion： The method is reliable and accurate with good reproducibility. The established fingerprints could be used as one of the quality control index for Jingjie （Herba schizonepetae）.