乙酰甲喹对大肠埃希氏菌的抗生素后效应试验观察

Observations of Postantibiotic Effect Ofmaquindox on E.Coli

试验观察乙酰甲喹对大肠埃希氏菌临床分离株的作用效果,测定乙酰甲喹的抗生素后效应(PAE),采用标准肉汤试管二倍稀释法测定药物对分离菌株的最小抑菌浓度(MIC),并在MIC的基础上采用菌落计数法分别测定0.15,1,2,4倍于MIC时对试验菌的PAE。结果显示,MIC值为2μg/mL,在药物质量浓度为0.5,1,2,4倍MIC时对分离菌株的PAE分别是0.5,1,2,3 h,表明乙酰甲喹对大肠埃希氏菌临床分离株有着明显的抑制作用,并存在PAE,且PAE具有浓度依赖性,随药物浓度升高,PAE的时间延长。

The purpose of this study is to observe the antimicrobial effect and the postantibiotic effect （PAE） of maquindox to the E. coli that isolated from veterinary clinic. The double broth dilution method is adopted to detect the minimum inhibitory concentration （MIC） of maquindox in the experiments, and also the PAE of 0.15, 1, 2, 4 times of MIC to the bacteria is detected by using colony counting method. The results show that the MIC of maquindox is 2 μg/mL and the PAE of the different concentration are 0.5, 1, 2 and 3 h, respectively. It suggests that the E. coli isolated in the experiments is resistant to maquindox. Moreover, the PAE of maquindox to the bacteria is elonged according to the increasing of its concentration, to E. coli isolated from clinic case has an obvious PAE. The PAE is able to prolong following the increase of drug concentration.