食品中柑橘红2号与罗丹明B及苏丹红Ⅰ-Ⅳ的高效液相色谱检验方法

High Performance Liquid Chromatography （HPLC） Testing Method of Citrus Red 2, Rhodamine B, Sudan Red Ⅰ-Ⅳ in Food

建立高效液相色谱同时测定食品中多种脂溶性染料的含量测定,脂溶性着色剂用正己烷提取后经中性氧化铝固相萃取柱净化,用反相色谱柱分离,高效液相色谱仪测定,外标法定量。样品前处理简单快捷,分离度高,灵敏度高且结果准确可靠,大大提高了实验室的工作效率。

High performance liquid chromatography （HPLC） is established at the same time the determination of food in a variety of content determination of fat soluble dyes, fat soluble colorant extracted with n-hexane after purification by neutral alumina solid-phase extraction column, using reversed phase chromatographic column separation, high-performance liquid chromatograph, external standard method. Sample preparation is simple and fast, high degree of separation, high sensitivity and accurate and reliable, greatly improving the efficiency of the laboratory.