摘要
目的利用响应面分析法对检验尿液中微量清蛋白以及血液中糖化血红蛋白的条件进行优化。方法随机选择60例糖尿病患者分别抽取清晨空腹静脉血3mL,同时留取尿标本6mL,对不同保存温度、保存时间进行单因素实验。根据中心组合Box-Behnken的实验设计原理,在单因素实验的基础上,对样品保存温度、保存时间2个影响显著因素采用双因素双水平的响应面分析法优化检验条件,以保存温度4℃、保存时间3d为中心点进行响应面分析实验。结果尿液以及血液测量的最佳保存温度是4℃,最佳保存时间是1d,在此条件下尿液中微量清蛋白以及血液中糖化血红蛋白的测量值几乎没有影响,十分接近于稳定情况下的理想值。结论利用响应面分析法可以优化微量清蛋白及糖化血红蛋白检验条件。
Objective To optimize the measurements of glycosylated hemoglobin and urine trace albumin based on the response surface methodology.Methods 60 cases of patients with diabetes were enrolled.All the patients were collected 3mL fasting blood and 6mL urine in the morning.The simple factors experiment was taken in different maintain temperatures and times.Based on the Box-Behnken design principles,the temperatures and times were as the factors influencing.The response surface methodology was proceeding centered on the condition that the maintain temperature was 4℃and the maintain time was 3d.Results The best temperature for save urine and blood was 4℃and the best time was 1d.In this condition,the measured value of glycosylated hemoglobin and urine trace albumin were mostly closed to the ideal value.Conclusion Using the response surface methodology,the measure condition of glycosylated hemoglobin and urine trace albumin can be optimized.
出处
《国际检验医学杂志》
CAS
2014年第21期2929-2930,2933,共3页
International Journal of Laboratory Medicine
关键词
响应面分析法
微量清蛋白
糖化血红蛋白
response surface methodology
trace albumin
glycosylated hemoglobin