全自动血液分析仪检测特殊体液有核细胞与手工法的结果比对

Comparison of the karyote detection results of automatic blood analyzer and manual method in special body fluids

目的比对Sysmex XE-5000全自动血液分析仪（XE-5000）与手工法检测特殊体液有核细胞计数与分类的结果。方法100份特殊体液（脑脊液、浆膜腔积液）标本同时用XE-5000与传统的显微镜手工法进行有核细胞计数与分类。结果当有核细胞计数为31-1 000/μL时,2种方法检测有核细胞计数的结果差异无统计学意义（P〉0.05）,并且检测的有核细胞计数和有核细胞分类结果具有相关性（r分别为0.981和0.991,P〈0.05）。结论当有核细胞计数为31-1 000/μL时,用XE-5000检测特殊体液有核细胞的准确性好、精密度高。

Objective To compare the karyote counting and classification results of Sysmex XE-5000 automatic blood analyzer（XE-5000）and manual method in special body fluids.Methods 100 cases of special body fluid specimens（including cerebrospinal fluid and serous cavity effusion）were analyzed,and the the karyote counting and classification were respectively detected by XE-5000 and the traditional manual method of microscope.Results When the karyote counts were 31-1 000/μL,there was no significant difference between XE-5000 and manual method（P〉0.05）.And the karyote counting and classification results of XE-5000 correlated with those of manual method（r=0.981,0.991,P〈0.05）.Conclusion When the karyote counts are 31-1 000/μL,XE-5000 has good accuracy and high precision in karyote detection in special body fluids.