基于WS/T406标准的血液分析仪比对实验

Comparison of different hematology analyzers according to WS/T406 standard

目的了解4台血液分析仪(LH780、LH750、XN-1000、XS-1000i)检测结果的符合程度。方法在每台仪器的批内精密度和批间精密度均符合要求的基础上,选取20份不同浓度的血常规临床标本,分别在各台仪器上测定。以LH750作为参比仪器,其测定值为标准值,计算另外3台仪器白细胞计数(WBC)、红细胞计数(RBC)、血红蛋白(Hb)、红细胞比容(HCT)、血小板计数(PLT)、红细胞平均体积(MCV)、红细胞平均血红蛋白量(MCH)、红细胞平均血红蛋白浓度(MCHC)等项目测定值与标准值的相对偏差及比对符合率。调查82份低值WBC标本、86份低值PLT标本以及35份有"血小板凝块"报警的标本的WBC和PLT的比对结果。结果 LH780各个项目的比对符合率均达到90%以上。XN-1000和XS-1000i的HCT比对符合率为80%,MCV和MCHC比对符合率低于80%,其余项目比对符合率达85%以上。调整了XN-1000和XS-1000i的HCT校正系数后,2台仪器的HCT、MCV和MCHC符合率达85%以上。WBC<1.0×109/L、PLT<30×109/L的标本WBC和PLT比对符合率在70%以下。有"血小板凝块"报警的标本中,WBC和PLT比对结果也不能达到相对偏差标准。结论应定期对同一实验室中的多台血液分析仪进行比对实验。

Objective To understand the result coincidence of 4hematology analyzers（LH780,LH750,XN-1000,and XS-1000i）.Methods 20 samples of routine blood test were selected and detected by different hematology analyzers whose within-run precision and between-run precision all met the requirements of WS/T 406 standard.LH750was chose to be the reference instrument,and its detection results were standard values.The relative deviations and coincidence rates of WBC,RBC,Hb,HCT,PLT,MCV,MCH and MCHC of the other 3hematology analyzers were calculated.The WBC and PLT coincidence results of 82 cases of low-WBC samples,86 cases of low-PLT samples,and 35 cases of platelet aggregation samples were investigated.ResultsThe coincidence rates of all items were more than 90%between LH780 and LH750.For XN-1000/XS-1000 i,the coincidence rate was 80%in HCT,and which were less than 80%in MCV and MCHC,and more than 85%in other items.After readjusting the calibration factors of HCT of XN-1000 and XS-1000 i,the coincidence rates of HCT,MCV and MCHC were all increased to more than 85%.The coincidence rates of WBC and PLT were less than 70%in low-WBC samples（WBC〈1.0×10^9/L）and low-PLT samples（PLT〈30×10^9/L）.The results of WBC and PLT could not reach the relative deviation standards in 35 platelet aggregation samples.Conclusion The comparative tests should be carried out among different hematology analyzers in one laboratory.