0.5%哈蟆油匀浆对NK细胞活性和细胞免疫功能影响实验研究

Study of NK Cell Activity and Cell-Mediated Immunity Effect on 0.5% of Oviductus Ranae Homogenate

目的:观察0.5%(g/mL)哈蟆油匀浆对NK细胞活性和增强细胞免疫作用的影响。方法:用乳酸脱氢酶LDH测定法研究对NK细胞活性的影响;用小鼠迟发型变态反应(DTH)研究对细胞免疫功能的影响。结果:经口给予小鼠不同剂量的0.5%哈蟆油匀浆30 d,对小鼠NK细胞活性无影响;能增强小鼠迟发型变态反应,即能增强细胞免疫功能。结论:0.5%哈蟆油匀浆对NK细胞活性无影响,根据动物实验结果,成人每日服用20 mL具有增强细胞免疫的作用。

Objective：To study NK cell activity and cell-mediated immunity effect of 0.5%（g/mL） of Oviductus Ranae homogenate.Methods：With LDH measurement,we studied the effect of 0.5% of Oviductus Ranae homogenate on mice NK cell activity.With DTH,we studied the effect of 0.5% g/mL of Oviductus Ranae homogenate on mice cell-mediated immunity.Results：Mice took different doses of 0.5% of Oviductus Ranae homogenate after 30 days,and there was no influence on NK cell activity of Oviductus Ranae.It can enhance mice delayed allergy.In other words,it can strengthen cell-mediated immunity in mice.Conclusion：0.5% of Oviductus Ranae homogenate has no influence on NK cell activity and according to the animal experimental results we calculated that daily use 0.5% of Oviductus Ranae homogenate 20 mL for adults can strengthen cell-mediated immunity.