菟丝子对大鼠肾脏缺血再灌注的保护作用

Protective Effect of Cuscuda chinensis Lam. Extract on Renal Ischemia-Reperfusion Injury

目的:考察菟丝子对大鼠肾脏缺血再灌注的保护作用,并探讨其作用机制。方法:30只大鼠按随机数表法随机分为空白对照组、模型组和菟丝子给药组,给药1周后,空白对照组进行假手术,模型组和菟丝子给药组建立大鼠肾脏缺血再灌注模型后150 min,检测血尿素氮、血清肌酐、丙二醛、超氧化物歧化酶和膜流动性。结果:模型组相对于空白对照组,SCR、BUN、MDA、SOD、荧光偏振度P和微黏度η均有显著性统计学差异(P<0.01);菟丝子给药组相对于空白对照组,SCR、BUN、MDA、SOD、荧光偏振度P和微黏度η均有显著性统计学差异(P<0.01);菟丝子给药组相对于模型组,SCR、BUN、MDA、SOD和微黏度η均有显著性统计学差异(P<0.01),荧光偏振度P有统计学差异(P<0.05)。结论:菟丝子能够预防护肾脏缺血后的再灌注损伤,其作用机制可能与菟丝子提高SOD、改善线粒体膜流动性和清除氧自由基有关。

Objective ： To study the protective effect of Cuscuda chinensis Lam. extract on renal ischemia reperfusion in rats and to explore its mechanism. Methods： 30 rats were randomly divided into blank control group, model group and Cuscuda chinensis Lam administration group. After treatment for 1 week, the blank control group received sham operation. The model group and Cuscuda chinensis Lain administration group established rat renal ischemia reperfusion model. After 150 min,blood urea nitrogen, serum creatinine, malondialdehyde, superoxide dismutase and membrane were detected. Results： The SCR, BUN, MDA, SOD, P fluorescence polarization and the microviscosity in model group compared with those of blank control group, there was significant statistical difference （P 〈 0.01 ）. When Cuscuda chinensis Lam administration group compared with the control group, SCR, BUN, MDA, SOD, P fluorescence polarization and the microviscosity were significant in statistics （ P 〈 0. 01 ）. When Cuscuda chinensis Lain administration group compared with model group, SCR, BUN, MDA, SOD and microviscosity had significant statistical difference （ P 〈 0.01 ） and P fluorescence polarization had significant difference （P 〈 0. 05）. Conclusion： Cuscuda chinensis Lain could prevent renal ischemia reperfusion injury and its mechanism may be related to increasing SOD and improving mitochondrial membrane fluidity and removal of oxygen free radicals.