正交试验优化麝香中多肽类成分的提取工艺

Optimization of Extraction Process of Polypeptides from Moschus by Orthogonal Design

目的:优选麝香总多肽的研磨提取工艺,为麝香的品种鉴定与质量评价提供参考。方法:采用福林酚法测定麝香总多肽含量。以麝香总多肽含量为指标,通过正交试验考察提取时间、溶媒种类及用量对提取工艺的影响。结果:最佳提取工艺为三羟甲基氨基甲烷-盐酸缓冲液(p H 8.0,0.025 mol·L-1),料液比1∶100,研磨时间3 h,放置过夜;麝香总多肽平均质量分数达20.71%,经冷冻干燥技术处理后麝香总多肽收率62.4%。结论:优选的提取工艺稳定可行,获得麝香总多肽的提取率较高,为麝香的品质分析提供实验依据。

Objective： To optimize extraction process of total polypeptides from Moschus and provide a reference for species identification and quality evaluation of Moschus. Method： The content of total polypeptides was determined by Folin phenol method. With the content of total polypeptides as index, orthogonal test was adopted to optimize extraction process by taking extracting time,the amount and type of solvent as factors. Result：Optimum extraction process of total polypeptide was as follows： with Tris-HCl buffer（ p H 8. 0,0. 025 mol·L^- 1）as extracting solution,grinding time of 3 h,solid-liquid ratio of 1 ∶ 100; the content of total polypeptides was20. 71%,after freeze-drying,yield of total polypeptides was 62. 4%. Conclusion： Optimized extraction process is stable and feasible with high extracting rate,this method can provide experimental basis for quality analysis of Moschus.