HPLC同时测定虎贞痛风胶囊中大车前苷、虎杖苷、特女贞苷、白藜芦醇的含量

Simultaneous Determination of Plantamajoside,Polydatin,Specnuezhenide and Resveratrol in Huzhentongfeng Capsule with HPLC

目的：建立同时测定虎贞痛风胶囊中大车前苷、虎杖苷、特女贞苷、白藜芦醇含量的HPLC方法。方法：采用高效液相法测定,色谱条件为Agilent SB-C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.1%甲酸溶液梯度洗脱,检测波长280 nm,流速1.0 m L·min-1,柱温30℃。结果：大车前苷、虎杖苷、特女贞苷、白藜芦醇分别在0.099 6-1.992（r=0.999 9）,0.123-2.46（r=0.999 9）,0.145-2.90（r=0.999 9）,0.025-0.50μg（r=0.999 8）与峰面积呈良好的线性关系,平均回收率分别为99.27%,99.43%,98.83%,98.07%。结论：该方法快速简便,重复性好,专属性强,可作为虎贞痛风胶囊的质量控制方法。

Objective： The aim of this study was to establish a HPLC method for simultaneous determination of the contents of plantamajoside,polydatin,specnuezhenide and resveratrol in Huzhentongfeng capsules. Method： HPLC was used to determine the contents. The analytical column was Agilent SB-C18column（ 4. 6 mm × 250 mm,5 μm）. The liquid phase was composed of acetonitrile and 0. 1% formic acid with gradient elution. The detection wavelength was set at 280 nm,the flow rate was 1. 0 m L·min- 1and the column temperature was maintained at 30 ℃. Result： The mass of plantamajoside,polydatin,specnuezhenide and resveratrol with peak areas were within the concentration ranges of 0. 099 6-1. 992 μg（ r = 0. 999 9）,0. 123-2. 46 μg（ r = 0. 999 9）,0. 145-2. 90 μg（ r = 0. 999 9）,0. 025-0. 50 μg（ r = 0. 999 8）,and the average recoveries were 99. 27%,99. 43%,98. 83%,98. 07%,respectively. Conclusion： The method is rapid and simple,highly reproducible and specific so that it could be used as one of the quality control methods of Huzhentongfeng capsules.