HPLC测定脱脂紫苏子中迷迭香酸和木犀草素的含量

Determination of Rosemary Acid and Luteolin in Defatted Perillae fructus by HPLC

目的：建立HPLC同时测定脱脂紫苏子中迷迭香酸和木犀草素含量的方法。方法：采用Diamonsil C18色谱柱（4.6 mm×250 mm,5μm）,流动相甲醇-0.1%磷酸溶液（55∶45）,流速1 m L·min^-1,检测波长330,350 nm,柱温30℃。结果：迷迭香酸在9.91-158.55 mg·L^-1回归方程为Y=108.83X＋0.14（r=0.999 9）,平均回收率为99.68%,RSD 2.01%;木犀草素在0.997-15.952 mg·L^-1回归方程为Y=119.63X-3.68（r=0.999 9）,平均回收率为100.34%,RSD 2.86%。结果：该方法操作简便、准确,重复性良好,可用于测定脱脂紫苏子中迷迭香酸和木犀草素的含量。

Objective： The aim of this study was to establish an HPLC method for simultaneous determination of the contents of Rosemary acid and Luteolin in defatted Perilla. Method： Diamonsil C18（ 4. 6mm × 250 mm,5 μm） column was used,with methanol-0. 1% phosphoric acid（ 55 ∶ 45） as mobile phase. The flow rate was set at 1. 0 m L·min^-1,the detection wavelength was set at 330 and 350 nm,with the column temperature at 30 ℃. Result： The linear response ranges were 9. 91-158. 55 mg·L^-1with regression curve Y =108. 83 X ＋ 0. 136 8（ r = 0. 999 9） for rosemary acid,and 0. 997-15. 952 mg · L^-1with regression curve Y =119. 63X- 3. 677 8（ r = 0. 999 9） for luteolin. The average recovery of rosemary acid and luteolin were 99. 68%and 100. 34%,respectively（ RSD 2. 01%,2. 86%）. Conclusion： A simple,accurate and reproducible HPLC method has been provided for simultaneous determination of rosemary acid and luteolin in defatted Perilla.