苦豆子种子生物碱类化学成分及细胞毒活性

Alkaloids Constituents of Sophora alopecuroides Seed and Their Cytotoxic Activity

目的：研究苦豆子种子的化学成分并对其抗肿瘤活性进行测试。方法：采用常规的硅胶柱层析（CC）,葡聚糖（Sephadex LH-20）柱层析,和重结晶等实验手段对其进行了分离纯化,利用波谱技术（1H-NMR,13C-NMR）及与文献数据对照,对分离的化合物进行了结构鉴定;并采用MTT测定部分生物碱单体化合物体外对C6肿瘤细胞的细胞毒活性。结果：共分离出7个生物碱类化合物,分别鉴定为：苦参碱（1）,氧化苦参碱（2）,槐果碱（3）,9α-羟基槐果碱（4）,氧化槐果碱（5）,9α-羟基槐胺碱（6）,（-）-13,14-去氢槐定碱（7）,1个非生物碱化合物3-吲哚甲醛（8）。结论：化合物8为首次从该属植物中分离得到。细胞毒活性测试结果表明,化合物1-3和5对鼠脑胶质瘤C6细胞显示了较强的生长抑制活性。

Objective： To investigate the chemical constituents in seed of Sophora alopecuroides and to determine their antitumor activities in vitro. Method： Compounds were isolated and purified by means of silica gel and Sephadex LH-20 gel columm chromatography,and recrystallization etc. Their structures were elucidated using spectroscopic analysis including1H-NMR,13C-NMR and spectral data. The cytotoxic activities of the alkaloids compounds on C6 cell in vitro were evaluated by MTT method. Result： Seven compounds were isolated from the ethanol extract of the seed of S. alopecuroides. Their structrues were identified as（ ＋）-matrine（ 1）,（ ＋）-oxymatrine（ 2）,（-）-sophocarpine（ 3）,（-）-9α-hydroxysophocarpine（ 4）,（ ＋）-oxysophocarpine（ 5）,（-）-9α-hydroxysophoramine（ 6）,（-）-13,14-dehydrosophoridine（ 7）,and a non-alkaloid named indolyl-3-carbaldehyde（ 8）. Conclusion： The compond 8 was isolated from the genus sophora for the first time.Compounds 1-3 and 5 showed significant inhibitory activity towards C6 glioma cells.