微电流检测和EBER原位杂交联合应用在鼻咽癌病理诊断中的意义

Application of pathological diagnosis of nasopharyngeal carcinoma by combining micro-current detector and EBER ISH

目的研究微电流值在鼻咽癌组织中的变化特点及EBER在鼻咽癌的表达情况。探索微电流检测和EBER原位杂交联合应用在鼻咽癌病理诊断中的意义。方法应用微电流检测和EBER分子原位杂交方法检测48例鼻咽癌、19例鼻咽黏膜慢性炎症,分析微电流值在鼻咽癌组织中的变化特点及EBER在鼻咽癌的表达情况。结果微电流检测的48例鼻咽癌中40例阳性(微电流值>0μA),阳性率为83.3%,19例鼻咽黏膜慢性炎症组织中3例阳性(微电流值>0μA),假阳性率15.8%;EBER原位杂交检测的48例鼻咽癌中38例呈阳性反应,阳性率为79.2%,19例鼻咽黏膜慢性炎症组织均阴性;微电流检测和EBER原位杂交联合检测48例鼻咽癌中44例阳性,阳性率为91.7%,差异有统计学意义。结论 1微电流检测和EBER原位杂交检测方法是较好的鼻咽癌病理诊断方法。2微电流检测和EBER原位杂交在鼻咽癌病理诊断中有相互补充作用,联合应用两种方法,可以提高鼻咽癌诊断正确率,减少假阴性率。

【Objective】 To research the micro current value and expression of EBER in nasopharygeal carcinoma and explore micro-current detector and EBER in situ hybridization joint application to diagnose nasopharygeal carcinoma. 【Methods】 48 cases of nasopharygeal carcinoma and 19 cases of nasopharyngeal chronic inflammation were detected by micro-current detector and EBER in situ hybridization and then the results of micro current value and expression of EBER in nasopharygeal carcinoma were analyzed. 【Results】40 cases（83.3%） of nasopharygeal carcinoma were positive expression detected by micro-current detector, 3cases（15.8%） of nasopharyngeal chronic inflammation were positive expression detected by micro-current detector; 38 cases（79.2%） of nasopharygeal carcinoma were positive expression detected by EBER in situ hybridization, 19 cases of nasopharyngeal chronic inflammation were negative expression detected by EBER in situ hybridization. 44 cases（91.7%） of nasopharygeal carcinoma were positive expression detected by micro-current detector and EBER in situ hybridization joint application, there were significant differences. 【Conclusion】 Micro-current detector and EBER in situ hybridization are better pathological diagnosis indicators. Micro-current detector and EBER in situ hybridization joint application to diagnose nasopharygeal carcinoma can improve diagnostic accuracy and reduce the false negative rate.