一个抗病性增强的水稻类病变突变体的蛋白质组学研究

A Proteomic Study on a Disease-Resistance-Enhanced Rice Lesion Mimic Mutant

水稻类病变突变体chl1具有抗病性增强的类病变表型,对水稻白叶枯病和稻瘟病都具有很强的抗性。利用蛋白质组学技术分析chl1与其野生型之间的差异表达蛋白,探讨chl1类病变表型的形成和抗病反应的分子机制。利用荧光双向差异凝胶电泳(two-dimensional fluorescence difference gel electrophoresis,2D-DIGE)技术和质谱分析,chl1中共鉴定到70个差异表达的蛋白点,包括46个上调蛋白点和24个下调蛋白点。这些蛋白点参与不同的生物过程,包括防御相关、光合作用、氧化还原、氨基酸/蛋白质代谢、分子伴侣、碳水化合物代谢。对这些差异表达蛋白进行生物信息分析,推测它们所在的复杂调控网络可能参与chl1叶片细胞程序性死亡(programmed cell death,PCD)及其抗病性的调控。

A lesion-mimic mutant in rice （Oryza sativa L.）,chloroplastic-H 2 O 2-induced lesion 1 （chl1 ）,has enhanced resistance to rice blast and bacterial blight.To understand the molecular mechanisms underlying this phenotype and its resistance responses,a proteomics-based approach was used to identify differentially-expressed proteins between chl1 and its wild type.Using two-dimensional fluorescence difference gel electrophoresis technology and mass spectrometry, 70 protein spots were successfully identified,of which 46 were up-regulated and 24 were down-regulated in the mutant. These differentially-expressed proteins are involved in diverse biological processes including disease resistance,photo-synthesis,oxidation-reduction reaction,amino acid/protein metabolism,chaperoning and carbohydrate metabolism. The complex regulatory network in which these proteins are involved may play an important role in regulating the pro-grammed cell death and the resistance reaction in chl1 .