白花蛇舌草抑制肝癌H22小鼠淋巴管转移的实验研究

Experimental Study on Inhibitory Effect of Hedyotis Diffusa Willd on Lymphatic Metastasis of Mice with Hepatocarcinoma H22

目的:观察白花蛇舌草对小鼠肝癌淋巴管转移的影响。方法:在小鼠右下肢爪垫内侧皮下注射接种腹水型肝癌H22瘤株,复制淋巴管转移模型。将模型小鼠随机分为5组,空白组每天灌生理盐水,大、中、小剂量组每天灌白花蛇舌草水煎液,对照组给予环磷酰胺腹腔注射。观察小鼠体质量、免疫器官、肺部转移率、爪垫移植瘤、淋巴结转移瘤等指标变化。结果:白花蛇舌草作用组的小鼠,体质量和免疫器官胸腺质量明显增长,肺部瘤结节数和肺部转移瘤生长指数均下降,且淋巴结转移数明显减少。结论:白花蛇舌草对H22肝癌淋巴管转移有一定抑制作用。

Objective：To observe effects of Hedyotis diffusa Willd in treating hepatocarcinoma lymphatic metastasis of mice. Methods：The H22 lymphatic metastasis models were established by subcutaneous injecting H22 cell line into the palmula of right lower limb. Th~ model mice were randomly divided into 5 groups. The blank group was given normal saline daily,and large,medium,small-dose group were given Hedyotis diffusa Willd,and the control group were received cyclophosphamide by intraperitoneal injection daily. The changes of mouse＇s body weight,the weight of immune organ,lung metastasis,paw pads transplantation tumor and lymph node metastasis were observed. Results：In Hedyotis diffusa Willd group,body weight and the weight of immune organs increased ,lung nodules and lung metastasis tumor growth index were decreased ,and the number of lymph node metastases was reduced. Conclusion：Hedyotis diffusa Willd has an inhibitory effect on the metastasis of hepatocarcinoma lymphatic.