摘要
[目的 ]调查贵州省马群马流感病毒、马疱疹病毒1/4型或马动脉炎病毒感染情况。[方法 ]用酶联免疫吸附试验(ELISA)对采自贵州省9个市(州)及第九届民运会参赛马匹的363份血清进行了A型流感病毒、马疱疹病毒1/4型、马动脉炎病毒和非洲马瘟病毒抗体检测。[结果 ]结果显示这四种病原的血清抗体群阳性率分别为79.49%(31/39)、7.69%(3/39)、43.59%(17/39)和0%(0/39),贵州本地役用马和赛马差异不显著(P>0.05);血清抗体个体阳性率分别57.85%(210/363)、1.38%(5/363)、11.29%(41/363)和0%(0/363),其中贵州本地役用马阳性率分别为38.16%(87/228)、4.39%(1/228)、8.33%(19/228)和0%(0/228),赛马阳性率分别为91.11%(123/135)、2.96%(4/135)、16.30%(22/135)和0%(0/135)。相比之下,A型流感病毒及马动脉炎病毒抗体阳性率存在差异(P<0.01或0.01<P<0.05),马疱疹病毒1/4型病毒抗体阳性率差异不显著(P>0.05)。[结论 ]调查表明贵州省部分马群存在不同程度的马流感病毒、马疱疹病毒1/4型或马动脉炎病毒感染。
Serological Investigation of Infections with Equine Infl uenza Virus,Equine Herpesvirus Type 1/4,Equine Arteritis Virus and African Horse Sickness Virus in Guizhou Province. 363 equine serum samples taken from 31 working and 8 racing horse herds in Guizhou Province were examined for antibodies against equine infl uenza virus（EIV）, equine herpesvirus type 1/4（EHV-1/4）,equine arteritis virus（EAV） and African horse sickness virus（AHSV） by enzyme-linked immunosorbert assay（ELISA）.The results showed that the positive rates of the four pathogens in the sampled horses were 57.85%（210/363）,1.38%（5/363）,11.29%（41/363）and 0%（0/363）and the positive rates in herds were 79.49%（31/39）,7.69 %（3/39）,43.59%（17/39）and 0%（0/39）respectively.The local working horses were different from racing horses in the positive rate of infl uenza A virus antibody（P 〈 0.01） and of equine arteritis virus antibody（0.01 〈 P 〈 0.05）,while the positive rates of herds and the total positive rate of equine herpes virus type 1/4 virus antibody were not signifi cant（P〉0.05）.
出处
《中国动物检疫》
CAS
2014年第11期86-89,共4页
China Animal Health Inspection
基金
贵州省科学技术基金项目"适量采集
安全处理重大动物疫病样品的方法研究"(20102114)
