补肝健腰方对腰椎退变大鼠椎间盘组织AQP1表达的影响

Effect of Bugan Jianyao Recipe on AQP1 Expression in Intervertebral Discs of Rats with Lumbar Disc Degeneration

目的:观察补肝健腰方对腰椎退变大鼠椎间盘组织水通道蛋白1(AQP1)表达的影响。方法:将60只大鼠随机分成3组,造模组35只、假手术组15只、正常对照组10只。造模成功后分组,分别给予补肝健腰方、腰腿痛丸或生理盐水喂养。分别于术后20天、40天和60天收集造模节段椎间盘组织,RT-PCR检测AQP1在椎间盘组织中的表达,免疫组化试剂检测AQP1表达与分布。结果:各组AQP1表达比较,假手术组与正常对照组比较无差异(P>0.05),模型组、腰腿痛丸组、补肝健腰方组与正常对照组比较均有差异(P<0.05),在术后20天、40天、60天补肝健腰方组与腰腿痛丸组比较,差异均有显著性意义(P<0.05),术后40天腰腿痛丸组、补肝健腰方组与同组术后20天比较,差异均有显著性意义(P<0.05)。结论:大鼠腰椎间盘退变后,AQP1的表达明显下降。在补肝健腰方的干预下,退变椎间盘细胞AQP1的表达有明显上调,推测AQP1的表达增减在椎间盘退变过程中起着重要的作用,补肝健腰方对腰椎间盘退变有预防及治疗作用。

Objective： To explore the effect of Bugan Jianyao recipe（B JR） on the expression of aquaporin（AQP）l in the intervertebral discs of rats with lumbar disc degeneration. Methods. Sixty rats were randomly divided into three groups, namely operation group with 35 rats, sham operation group with 15 rats and normal group with 10 rats. After successful establishment of the model, rats in the operation group were divided into model group, B JR group and the Lumbocrural Pain PilIs（LPP） group, which were treated with normal saline, B JR, LPP, respectively. The level of AQP1 mRNA was detected by Rt-PCR method and the expression of AQP1 was observed by immunohistochemical reagents 20, 40 and 60 days after operation. Results= No statistical significant difference was found in the expression of AQP1 between the sham operation group and the normal group（P 〉 0.05）. The difference of AQP1 expression between the normal group and the operation group was significant after treatment（P 〈 0.05）. The difference of AQP1 expression was also significant between BJD group and LPP group 20, 40 and 60 days after operation （P 〈 0.05）. AQP1 expression in BJD group and LPP group 40 days after operation differed from that in the same group 20 days after operation （P 〈 0.05）. Conclusion： AQP1 expression shows obvious decrease in rats with lumbar disc degeneration, and the intervention of B JR has an up-regulation of AQP1, indicating that AQP1 plays an important role in the development of intervertebral disc degeneration, and B JR has preventive and therapeutic effect on lumbar disc degeneration.