聚乙二醇化精氨酸脱亚胺酶的HPLC法测定

Determination of PEGylated Arginine Deiminase by HPLC

建立了分子排阻色谱-高效液相色谱法测定聚乙二醇化精氨酸脱亚胺酶（PEG-1）.使用凝胶色谱柱,以0.05 mol/L磷酸缓冲液（pH 7.3）∶甲醇（80∶20）为流动相,检测波长220 nm.PEG-1在0.2～1.2 mg/ml范围内线性关系良好.回收率为96.32％,RSD为1.74％.

A size exclusion chromatography （SEC）-HPLC method was established for the determination of the PEGylated arginine deiminase （PEG-1）.A gel column was used with the mobile phase of 0.05 mol/L phosphate buffer （pH 7.3） ∶ methanol （80 ∶ 20） at the detection wavelength of 220 nm.The calibration curve of PEG-1 was linear in the range of 0.2-1.2 mg/ml.The recovery was 96.32％,with RSD of 1.74％.