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亮甲酚蓝染色对牛卵母细胞体外成熟和凋亡的影响 被引量：2

Effect of BCB Staining on the IVM Apoptosis of Cow Oocytes
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摘要 为了探讨亮甲酚蓝(brilliant cresyl blue,BCB)对牛未成熟卵母细胞染色选择后对其体外成熟(IVM)和凋亡的影响,本研究在牛卵母细胞成熟培养前用26μmol/L BCB染色90min作为处理组,以未染色卵母细胞作为对照组;将处理组依照胞质的颜色分为蓝色组(BCB+)和无色组(BCB-),成熟培养后检测卵子的发育能力。结果表明:1经BCB染色卵母细胞在成熟后,BCB+组卵母细胞成熟率(80.92%)较对照组(60.00%)和BCB-组(51.61%)差异显著(P<0.05);2BCB+组的凋亡率(6.50%)较对照组(28.21%)、BCB-组(39.06%)差异显著(P<0.05),随着卵母细胞发育潜能的升高其凋亡率逐渐降低。由此可见,以亮甲酚蓝染色为基础的牛卵丘-卵母细胞复合体的区分可以用来有效的选择更具发育活力的牛卵母细胞。 The aim of this investigation was to study the effect of brilliant cresyl blue （BCB） staining on IVM apoptosis in cow, COCs without BCB staining was considered as control group, and COCs stained with BCB in 26 μmol/L for 90 min as treatment group before mature culture. Treated oocytes were divided into BCB^- （colourless cytoplasm） and BCB^＋ （coloured cytoplasm）. After IVM, to assess the developmental potential of the oocytes. The results showed that, the BCB^＋ oocytes had a significantly higher proportion of mature rate （80. 92 %） than control group （60. 00 %） and BCB^- oocytes （51. 61 %） （P〈 0. 05） ;The BCB^＋ oocytes yielded a significantly lower apoptosis rate （6.50%） than that in control （28. 21%） and BCB^- oocytes （39. 06%）（P〈0. 05）. These results suggested that the staining of cow cumulus-oocyte complexes with BCB before in vitro maturation could be used to select developmentally competent oocytes.
作者 乔利敏 乔富强 姚华 雷莉辉 王金秋 关伟军 马月辉
机构地区 北京农业职业学院畜牧兽医系 北京农学院科技产业集团 北京农学院动物科学技术学院 中国农业科学院北京畜牧兽医研究所
出处 《中国畜牧兽医》 CAS 北大核心 2014年第11期171-174,共4页 China Animal Husbandry & Veterinary Medicine
基金 北京农业职业学院博士基金项目:牛瘤胃补液器的设计制作与推广(XY-BS-12-04) 北京农业职业学院技术研发与示范推广基金项目:奶牛细管冻精解冻后的保存与应用研究(XY-YF-14-20)
关键词 牛 卵母细胞 亮甲酚蓝染色 体外成熟 cow oocytes brilliant cresyl blue staining IVM
分类号 Q813.1 [生物学—生物工程]
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参考文献13

  • 1乔利敏,李向臣,乔富强,姚华,张文秀,关伟军,马月辉.利用亮甲酚蓝染色法预测牛卵母细胞发育潜能的研究[J].畜牧兽医学报,2011,42(9):1249-1255. 被引量：1
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二级参考文献19

  • 1李媛,姜晶晶,马水英,李梅,胡京美,杨惠军,陈子江.未成熟卵母细胞评分系统对人类未成熟卵体外成熟及发育潜能的评价[J].生殖与避孕,2006,26(7):408-413. 被引量：6
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  • 9URDANETA A, JIMENEZMECEDO A R, IZQIERDO D, et a1.Effect of the addition of glutathione and glucose to the culture medium on embryo development of IVMIVF prepubortai goat oocytes[J].Zygote,2003,11(2):131-138.
  • 10URDANETA A , JIMENEZMECEDO A R, IZQIERDO D, et a1.Supplementation with cysteamine during maturation and embryo culture on embryo development of prepubertal goat oocytes selected by the brilliant eresyl blue test [J].Zygote, 2003,11(4):347-354.

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二级引证文献2

中国畜牧兽医
2014年 第11期

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