荧光定量PCR法检测乙肝病毒DNA和乙肝病毒标志物检测的临床价值分析

Analysis in value of fluorescence quantitative PCR in detection of HBV-DNA and HBV markers

目的探讨荧光定量PCR法(FQ-PCR)检测乙肝病毒DNA(HBV-DNA)和乙肝病毒标志物(HBV-M)ELISA法检测的临床价值。方法选取本院收治的乙肝患者653例,先采用ELISA法对其血液标本进行HBV-M模式定性检测,再采用FQ-PCR法进行HBV-DNA定量检测,观察不同HBV-M模式检测结果。结果大三阳(1、3、5模式)和1、3模式的HBVDNA阳性率分别为97.97%和94.74%,显著高于其他模式(P<0.05)。大三阳(1、3、5模式)的HBV-DNA表达水平为(5.59×106±2.42×105),显著高于其他模式(P<0.05)。结论不同HBV-M模式的HBV-DNA表达水平及阳性率存在显著差异,联合HBV-M定性及HBV-DNA定量检测对临床早期诊断及用药具有重要指导价值。

Objective To explore the clinical value of fluorescence quantitative PCR in detection of HBV-DNA and HBV markers.Methods 653 hepatitis B patients were collected.Firstly ELISA was used to detect HBV-M model of the blood samples,and then the FQ-PCR method was used to detect HBV-DNA.Results Positive rates of 1,3,5model and 1,3model of the HBV-DNA were 97.97%and 94.74%respectively,which were significantly higher than other model（P〈0.05）.1,3,5model of HBV-DNA expression was the highest（5.59×10^6＋2.42×10^5）,which was significantly higher than other model（P〈0.05）.Conclusion Different HBV-M patterns of HBV-DNA expression level and positive rate show significant differences.Combination of qualitative and quantitative detection of HBV-DNA and HBV-M shows agreat value in guiding of clinical early diagnosis and therapy.