ELISA方法检测牛组织中的阿维菌素类药物残留

An ELISA method to detect the residue of avermectins in bovine tissues

建立一种间接竞争ELISA方法,检测牛组织中的阿维菌素类药物(Avermectins,AVMs)残留。该方法可同时检测牛肝脏和肌肉中的阿维菌素、伊维菌素和埃普里诺菌素残留。标准曲线:y=-45.613x+81.191,R2=0.9951,IC50=4.8ng/mL,线性范围为1.1ng/mL^21.9ng/mL。空白牛肝脏组织中以20、50ng/mL和100ng/g浓度添加时,AVMs回收率为53.8%~80.4%,变异系数为3.4%~17.9%;空白牛肌肉组织中以5、10ng/mL和20ng/g浓度添加时,AVMs回收率为70.9%~108.6%,变异系数为3.7%~17.1%。

An ELISA method was developed to detect AVM,IVM,and EPR residues in bovine tissues. The formula was y =-45.613 x ＋ 81.191,R2= 0.9951,and the 50% inhibition concentration（IC50） was 4.8 ng/mL. The linear detecting range of calibrationcurve was from 1.1 ng/mL to 21.9 ng/mL. When the spiked concentrations of AVMs were 20 ng/g,50 ng/g, and 100 ng/g in bovine liv-er,the recovery of AVMs was 53.8%~80.4% with coefficients of variation（CV） of 3.4%~17.9%. When the spiked concentrations ofAVMs were 5 ng/g,10 ng/g,and 20 ng/g in bovine muscle,the recovery of AVMs was 70.9%~108.6% with CV of 3.7%~17.1%.