同时感染雏鹅的两种血清型沙门菌分离鉴定及遗传进化分析

Identification and Polygenetic Analysis of Two Serotypes of Salmonella Isolated from Gosling

为了解鹅源沙门菌的病原学特性,本研究从一发病雏鹅体内分离到2株细菌,根据培养特性、染色镜检、生化试验、PCR检测及血清学分型结果鉴定,分离菌分别为鼠伤寒沙门菌与印第安纳沙门菌。药敏试验表明,2个沙门菌分离株均为多重耐药菌株,其中鼠伤寒沙门菌耐3种药物(耐药率16.7%),而印第安纳沙门菌耐12种药物(耐药率66.7%);2个分离株均对头孢曲松钠、呋喃唑酮、多粘菌素B及链霉素表现高度敏感。对分离菌株16S r DNA基因进行扩增测序后,与Gen Bank登录的22株不同血清型沙门菌序列进行比较,构建系统进化树,结果显示:2个分离菌株具有较高同源性,与大多数泛嗜性血清型亲缘性较近,而与鸡沙门菌感染密切相关的血清型亲缘性相距较远。本研究为鹅沙门菌病的流行病学调查积累了数据,也为临床防控提供了试验依据。

To investigate the pathogenic characteristics of goose salmonellosis,two bacterial strains isolated from the sick gosling were identified as Salmonella Typhimurium and Salmonella Indiana by cultural characteristics,Gram＇s stainings,biochemical tests,PCR tests and serotyping.The drug susceptibility tests showed that both of them were muti-drug resistant but highly sensitive to ceftriaxone sodium,furazolidone,polymyxin B and streptomycin,Salmonella Typhimurium was resistant to 3 antibiotics（resistance rate 16.7%）,and Salmonella Indiana was resistant to 12 antibiotics（resistance rate 66.7%）.16 S r DNA gene of the isolates were amplified,and their sequences were compared to those of 22 different serotypes of Salmonella recorded in Gen Bank.The results revealed that two isolates were highly homologous and closer to most of pan-tropic serotypes,but had distant affinities with serotypes usually invaded chicken.This research provides available data to epidemiological studies of poultry salmonellosis,and simultaneously helps to prevent and control goose salmonellosis.