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高尔基体堆叠蛋白GRASP55结构折叠变化研究

Structural Diversification of the Golgi Stacking Protein GRASP55
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摘要 哺乳动物细胞中,高尔基体是由数个扁平囊泡堆叠形成的高度有极性的细胞器,其堆叠主要通过GRASP55和GRASP65蛋白的寡聚化来介导.GRASP55由N端两个串联的PDZ结构域和C端脯氨酸丰富结构域组成.前期工作中发现PDZ结构域末尾存在一段内部配体序列在GRASP55寡聚过程中有重要作用.为进一步验证该内部配体的作用,实验中以不含内部配体的截短型GRASP55为研究对象进行结构学研究,发现GRASP55中存在包含此内部配体的3处在构象上易变的区域,通过这些区域在折叠上的变化而影响GRASP55的寡聚化,进而影响高尔基体的堆叠. The Golgi apparatus is composed of a highly ordered parallel array of cisternae which form a stacked structure typically found in mammalian cells.The formation of Golgi apparatus is mediated mainly by the oligomerization of GRASP55 and GRASP65protein.GRASP55 consists of two connected N-terminal PDZ domain and C-terminal proline rich domain.Previous work identified the PDZ2 domain terminus contains a short internal ligand and plays an important role in the polymerization of GRASP55.To further verify the internal ligand function,truncated GRASP55 exclusive the internal ligand structure was selected as the investigate subject,and found containing the internal ligand totally three variable regions inGRASP55,these regions permute spatial structure and contribute on GRASP55 oligomerization,thereby effect on Golgi stacking.
出处 《南开大学学报(自然科学版)》 CAS CSCD 北大核心 2014年第5期31-35,共5页 Acta Scientiarum Naturalium Universitatis Nankaiensis
基金 国家重点基础研究发展计划(973计划)(2010CB911800)
关键词 高尔基体堆叠 GRASP55 晶体结构 构象变化 Golgi stacking GRASP55 crystal structure conformational changes
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参考文献16

  • 1王玲霞,刘志敏.蛋白的高尔基体定位[J].生物技术通讯,2008,19(1):115-119. 被引量:1
  • 2Xiang Y, Wang Y Z. GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking[J]. J Cell Biol, 2010, 188(2): 237--251.
  • 3Short B, Preisinger C, Korner R, et al. A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic[J]. J Cell Biol, 2001, 155(6): 877--83.
  • 4Marra P, Maffucci T, Daniele T, et al. The GM130 and GRASP65 Golgi proteins cycle through and define a subdo- main of the intermediate compartment[J]. Nat Cell Biol, 2001, 3(12): 1 101--1 113.
  • 5张照亮,鲍时来.高尔基体堆形成的分子机制[J].生物学通报,2006,41(11):4-5. 被引量:1
  • 6Feng Y, Yu W, Li X, et al. Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins [J]. The Journal of Biological Chemistry, 2013, 288(39): 28 418--28 427.
  • 7Otwinowski Z, Minor W. Processing of X-ray diffraction data collected in oscillation mode[J]. Macromolecular Crystallography, Pt A, 1997, 276: 307--326.
  • 8Winn M D, Ballard C C, Cowtan K D, et al. Overview of the CCP4 suite and current developments[J]. Acta crys- tallographica Section D, Biological Crystallography, 2011, 67: 235--242.
  • 9Emsley P, Cowtan K. Coot: Model-building tools for molecular graphics[J]. Acta Crystallogr D, 2004, 60:2 126-- 2 132.
  • 10Truschel S T, Sengupta D, Foote A, et al. Structure of the membrane-tethering GRASP domain reveals a unique PDZ ligand interaction that mediates golgi Biogenesis[J]. J Biol Chem, 2011, 286(23):20 125--20 129.

二级参考文献61

  • 1Adam D.Linstedt,Golgi complex: Stacking the cisternae,Current Biology 1999,9:R893-R896.
  • 2Vladimir Lupashin, Elizabeth Sztul,Golgi tethering factors,Biochimica et Biophysica Acta.2005,1744 : 325-339.
  • 3Yanzhuang Wang,Joachim Seemann,Marc Pypaert, James Shorter and Graham Warren A direct role for GRASP65 as a mitotically regulated Golgi stacking factor, The EMBO Journal 2003,22:3279-3290.
  • 4Yanzhuang Wang, Ayano Satoh, and Graham Warren, Mapping the Functional Domains of the Golgi Stacking Factor GRASP65, THE JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 280,No.6,Issue of February 2005,11:4921-4928.
  • 5A. Diao, D. Rahman,D.J. Pappin,J.Lucocq,M.Lowe, The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation,J.Cell Biol.2003,160:201-212.
  • 6Manojkumar A. Puthenveedu, Collin Bacher, Sapna Puri, Frederick Lannil and Adam D. Linsted, GM130 and GRASP65-dependent lat eral cisternal fusion allows uniform Golgi-enzyme distribution, Nat Cell Biol.2006,8(3):238-48.
  • 7Colley K J, Lee E U, Paulson J C. The signal anchor and stem regions of the β-galactoside α-2,6-sialyltransferase may each act to localize the enzyme to the Golgi apparatus [J]. J Biol Chem, 1992,267:7784-7793.
  • 8Fenteany F H, Colley K J. Multiple signals are required for alpha2,6-sialyhransferase(ST6Gall) oligomerization and Golgi localization[J]. J Biol Chem, 2005,280:5423-5429.
  • 9Machamer C E. Golgi retention signals: do membranes hold the key[J]? Trends Cell Biol, 1991,1:141-144.
  • 10Zambrano F, Fleischer S, Fleischer B. Lipid composition of the Golgi apparatus of rat kidney and liver in comparison with other subcellular organelles[J]. Biochim Biophys Acta, 1975,380:357-369.

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