摘要
哺乳动物细胞中,高尔基体是由数个扁平囊泡堆叠形成的高度有极性的细胞器,其堆叠主要通过GRASP55和GRASP65蛋白的寡聚化来介导.GRASP55由N端两个串联的PDZ结构域和C端脯氨酸丰富结构域组成.前期工作中发现PDZ结构域末尾存在一段内部配体序列在GRASP55寡聚过程中有重要作用.为进一步验证该内部配体的作用,实验中以不含内部配体的截短型GRASP55为研究对象进行结构学研究,发现GRASP55中存在包含此内部配体的3处在构象上易变的区域,通过这些区域在折叠上的变化而影响GRASP55的寡聚化,进而影响高尔基体的堆叠.
The Golgi apparatus is composed of a highly ordered parallel array of cisternae which form a stacked structure typically found in mammalian cells.The formation of Golgi apparatus is mediated mainly by the oligomerization of GRASP55 and GRASP65protein.GRASP55 consists of two connected N-terminal PDZ domain and C-terminal proline rich domain.Previous work identified the PDZ2 domain terminus contains a short internal ligand and plays an important role in the polymerization of GRASP55.To further verify the internal ligand function,truncated GRASP55 exclusive the internal ligand structure was selected as the investigate subject,and found containing the internal ligand totally three variable regions inGRASP55,these regions permute spatial structure and contribute on GRASP55 oligomerization,thereby effect on Golgi stacking.
出处
《南开大学学报(自然科学版)》
CAS
CSCD
北大核心
2014年第5期31-35,共5页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
国家重点基础研究发展计划(973计划)(2010CB911800)