富氢水预处理对大鼠肝缺血再灌注损伤的保护研究

The protective effects of hydrogen-rich suline preconditioning on hepatic is chemia-reperfiision injury in rats

目的探讨富氢水预处理对大鼠肝缺血再灌注损伤的影响。方法健康雄性SD大鼠30只,质量220~250 g,釆用随机数字表法分为3组（n=10）,假手术组（Sham组）、缺血再灌注组（IR组）和富氢水组（HS组）。Sham组仅暴露肝门,不夹闭动、静脉;IR组采用夹闭左、中叶肝肝动脉支60 min（保留门静脉血流）,然后恢复灌注,制备大鼠肝缺血再灌注模型;HS组于手术前3天口服2%富氢水（40 ml·kg-1·d-1）,其余两组口服同等剂量生理盐水。各组于再灌注6 h抽取下腔静脉血样并取肝组织,采用全自动生化分析仪测定血清谷丙转氨酶（ALT）和谷草转氨酶（AST）活性,光镜下观察肝组织病理学变化。结果与Sham组相比,IR组血清ALT和AST升高（P〈0.05）;与IR组相比,HS组ALT和AST活性降低（P〈0.05）;HS组肝病理学损伤较IR组明显减轻。结论硫化氢后处理可减轻大鼠肝缺血再灌注损伤。

Objective：To investigate the effects of hydrogen - rich suline postcondi - tioning on hepatic ischemia -reperfusion injury in rats. Methods：Thirty healthy male SD rats weighing 220 ~ 250g were randomly divided into 3 groups（n= 10 each）：Shame operaton group（ Sham group）,ischemia - reperfusion group（ IR group）and hydrogen - rich suline groups（HS groups）In Sham group hepatic portal was only exposed but not ligated. IR group the left and middle lobes of he-patic pedicle,portal vein and hepatic artery were clamped for 60 min and then reperfused. HS groups 3 days before the sur-gery were received oral 2% rich hydrogen - rich water（40 ml＆#183;kg - 1 ＆#183;d - 1 ）. Blood samples were collected for measuring the activity of alanine transaminase（ALT）and aspartate transaminase（AST）in serm at 6h after reperfusion. Liver tissue samples were collected for pathologic examination. Results ：Compared with Sham group,the activities of ALT and AST was increased,Compared with IR group,the activities of ALT and AST in serm was decreased. Hepatic tissue pathologic injury was reduced in HS groups as compared with IR group. Conclusion：Hydrogen - rich suline preconditioning can reduce hc-patic ischemia - reperfusion injury in rats.