白芦藜醇对大鼠类固醇糖尿病的影响及其作用机制研究

Effect and Action Mechanism of Resveratrol on Steroid-induced Diabetes Mellitus in Rats

目的:探讨不同剂量的白芦藜醇(Resveratrol)对大鼠类固醇糖尿病(SDM)的影响及机制。方法:采用SD大鼠随机分为正常对照组(NC组),类固醇糖尿病组(SDM组)和白芦藜醇低剂量组(LR组),中剂量组(MR组)和高剂量组(HR组);检测各组大鼠血糖,胰岛素抵抗,血清中脂联素和炎性因子肿瘤坏死因子-α(TNF-α)的改变,胰岛细胞凋亡率和胰腺组织细胞凋亡蛋白BCL2的变化。结果:与SDM组相比,MR组和HR组大鼠血糖、胰岛素抵抗、TNF-α的表达、胰岛细胞凋亡率明显降低(P<0.05),MR组和HR组大鼠血清脂联素和胰岛BCL2的表达明显升高(P<0.05)。结论:中剂量和大剂量白芦藜醇对大鼠SDM具有保护作用,这种保护作用与降低SDM大鼠血糖、抑制胰岛素抵抗、抑制胰岛炎性因子表达、上调增殖蛋白BCL2的表达,从而降低胰岛细胞的凋亡有关。

This article was aimed to study the effect of resveratrol on steroid-induced diabetes mellitus（SDM） in rats and the related mechanism. SD rats were randomly divided into the normal control（NC） group, SDM group, low dosage of resveratrol（LDR） group, middle dosage of resveratrol（MDR） group, and high dosage of resveratrol（HDR） group. All groups were tested on the change of blood glucose, insulin resistance（IR）, adiponectin and tumor necrosis factor-α（TNF-α） in serum. The rate of cellular apoptosis in pancreas islet and the expression of BCL2 in rats were also observed. The results showed that compared with the SDM group, the blood sugar, IR, TNF-α level, and the ratio of pancreas islet cellular apoptosis in serum of the MR group and HDR group were lower（P〈0.05）. The expression of adiponectin and BCL2 protein were up-regulated in the MR group and HDR group（P〈0.05）. It was concluded that resveratrol can protect rats against the injury from MDR and HDR of SDM. This protective effect is possibly mediated by down-regulating the blood glucose, IR, inflammation factors in pancreas islet, and up-regulation of BCL2 protein, in order to inhibit cellular apoptosis in pancreas islet.